Soybean Cultivar 2773054 Breeding via Gene Editing

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Solution Overview

Problem

Soybean breeders face challenges in developing stable, high-yielding soybean cultivars with desirable traits such as disease resistance, drought tolerance, and improved agronomic quality, as existing methods are time-consuming and unpredictable, and there is a need for efficient methods to introduce specific genetic traits like herbicide resistance and improved nutritional quality.

Innovation Solution

The development of soybean cultivar 2773054, which can be used in crossing and mutagenesis or transformation to produce plants with enhanced traits, including herbicide resistance, disease resistance, and improved nutritional quality, using methods like CRISPR-Cas9 gene editing, TALENs, and backcrossing, along with the creation of regenerable tissue cultures for uniform and stable production.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional breeding methods are used to develop soybean cultivars with desirable traits, then genetic diversity and trait combinations can be achieved, but the process is time-consuming and unpredictable

Engineering Contradiction:
Improvepredictability of trait inheritanceVSAvoidbreeding cycle duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces traditional mechanical breeding methods (crossing, selection, and recombination through physical means) with genetic engineering techniques (CRISPR-Cas9, TALENs, and transgenic transformation) that directly modify or introduce specific genes. This substitution enables precise control over trait inheritance, eliminating the unpredictability and time constraints of conventional breeding while achieving the same goal of developing soybean cultivars with desirable traits such as herbicide resistance and improved nutritional quality

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Adaptability or versatility

If multiple breeding steps are performed to combine desirable traits, then superior cultivars can be developed, but the complexity and number of steps increase

Engineering Contradiction:
Improvecombination of desirable traitsVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent replaces complex multi-step mechanical breeding programs with direct genetic modification techniques. Instead of performing numerous crosses, selections, and backcrosses to combine multiple desirable traits, the invention uses CRISPR-Cas9, TALENs, or transgenic methods to introduce or edit specific genes in a single step, thereby achieving the same trait combination goal with significantly reduced procedural complexity

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent changes the fundamental parameter of how traits are introduced into soybean cultivars. Rather than relying on gradual accumulation through repeated breeding cycles, the invention directly modifies genetic parameters by inserting, deleting, or editing specific DNA sequences, enabling rapid achievement of desired trait combinations without the complexity of traditional breeding programs

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS11185038B2Soybean cultivar 2773054
Publication Date: 2021.11.30 CONFLUENCE GENETICS LLC

AI summary

A soybean cultivar designated 2773054 is disclosed. Embodiments include the seeds of soybean 2773054, the plants of soybean 2773054, to plant parts of soybean 2773054, and methods for producing a soybean plant produced by crossing soybean 2773054 with itself or with another soybean variety. Embodiments include methods for producing a soybean plant containing in its genetic material one or more genes or transgenes and the transgenic soybean plants and plant parts produced by those methods. Embodiments also relate to soybean cultivars, breeding cultivars, plant parts, and cells derived from soybean 2773054, methods for producing other soybean cultivars, lines or plant parts derived from soybean 2773054, and the soybean plants, varieties, and their parts derived from use of those methods. Embodiments further include hybrid soybean seeds, plants, and plant parts produced by crossing 2773054 with another soybean cultivar.