Soybean 5PKGL32 Breeding via Molecular Marker Selection

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding procedures that are time-consuming and unpredictable, leading to significant research costs and variability in resulting soybean varieties.

Innovation Solution

The development of the soybean variety 5PKGL32, which incorporates specific genetic traits through crossing and backcrossing techniques, including the use of transgenic genes for herbicide resistance and disease resistance, and employs molecular marker-assisted selection to enhance breeding efficiency and consistency.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding procedures are used, then soybean varieties can be developed, but the process is time-consuming and unpredictable

Engineering Contradiction:
Improvepredictability of breeding outcomeVSAvoidbreeding time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent employs molecular marker-assisted selection that provides continuous feedback on the genetic composition of breeding plants. By monitoring specific DNA markers associated with desirable traits, breeders can make informed selection decisions at early stages, significantly improving predictability and reducing the time required to develop new varieties compared to conventional phenotypic selection methods.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The patent replaces conventional mechanical/phenotypic breeding methods with molecular biology-based techniques. Instead of relying on observable traits and traditional crossing methods, the invention uses DNA marker analysis to identify and select plants with desired genetic characteristics, thereby accelerating the breeding process and improving reliability.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Reliability

If conventional breeding procedures are used, then soybean varieties can be developed, but research costs are significant

Engineering Contradiction:
Improveconsistency of breeding resultsVSAvoidresearch cost
Core Design Contradiction:
ReliabilityVSLoss of energy

Solution Approach 1:

The patent implements preliminary genetic analysis using molecular markers at early breeding stages. By identifying plants with desirable genetic traits before phenotypic expression occurs, the method prevents investment in plants that would not meet breeding objectives, thereby reducing wasted resources and research costs while improving result consistency.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The invention substitutes expensive and time-consuming conventional breeding trials with more efficient molecular marker analysis. This replacement reduces the need for extensive field testing and multi-generation breeding programs, thereby lowering research costs while maintaining or improving result consistency.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Reliability

If transgenic genes are introduced for herbicide resistance, then disease and herbicide resistance are improved, but genetic complexity increases

Engineering Contradiction:
Improveresistance to diseases and herbicidesVSAvoidgenetic structure complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent extracts and introduces only the specific genetic elements necessary for desired traits (herbicide resistance genes and associated molecular markers). By focusing on individual, well-defined genetic components rather than entire genomes or complex trait clusters, the method achieves improved resistance while managing genetic complexity through targeted modification.

Inventive Principle:
Principle #2Taking out (Extraction)

Data Source

PatentUS10420312B1Soybean variety 5PKGL32
Publication Date: 2019.09.24 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 5PKGL32. Provided by the invention are the seeds, plants and derivatives of the soybean variety 5PKGL32. Also provided by the invention are tissue cultures of the soybean variety 5PKGL32 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 5PKGL32 with itself or another soybean variety and plants produced by such methods.