Soybean Variety A1026287 Breeding via Agrobacterium Transformation

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, leading to the need for innovative approaches to introduce specific genetic traits effectively.

Innovation Solution

The development of the soybean variety A1026287, which includes methods for crossing and breeding techniques like backcrossing, genetic transformation, and marker-assisted selection to introduce desired traits like herbicide resistance, disease resistance, and improved nutritional quality, along with the use of tissue culture and in vitro regeneration for efficient propagation and transformation.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding techniques are used, then soybean varieties can be developed with desirable traits, but the process is time-consuming and unpredictable

Engineering Contradiction:
Improvepredictability of trait introductionVSAvoidbreeding development time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces conventional mechanical breeding methods (crossing, selection, and regeneration in tissue culture) with biotechnological methods (genetic transformation using Agrobacterium or particle bombardment). This substitution introduces specific genetic traits directly into the soybean genome, eliminating the time-consuming and unpredictable nature of conventional breeding while maintaining the desired traits.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Productivity

If genetic transformation methods are used, then specific traits can be introduced efficiently, but the process complexity increases

Engineering Contradiction:
Improveefficiency of trait introductionVSAvoidbreeding process complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent uses Agrobacterium tumefaciens as an intermediary organism to transfer desired genetic traits into soybean cells. This natural biological vector simplifies the transformation process compared to direct DNA injection or particle bombardment, as Agrobacterium naturally transfers DNA through its Ti plasmid mechanism, reducing overall process complexity while maintaining high efficiency.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Adaptability or versatility

If multiple breeding techniques are combined, then superior varieties with multiple traits can be developed, but the manufacturing complexity increases

Engineering Contradiction:
Improvenumber of desirable traits in varietyVSAvoidbreeding process simplicity
Core Design Contradiction:
Adaptability or versatilityVSEase of manufacture

Solution Approach 1:

The patent employs marker-assisted selection during the breeding process to preliminarily identify and select plants that have successfully incorporated desired traits. This preliminary screening using molecular markers allows breeders to focus resources on promising candidates, simplifying the overall process of developing varieties with multiple traits by early elimination of non-conforming plants.

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS9155268B2Soybean variety A1026287
Publication Date: 2015.10.13 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated A1026287. Provided by the invention are the seeds, plants and derivatives of the soybean variety A1026287. Also provided by the invention are tissue cultures of the soybean variety A1026287 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety A1026287 with itself or another soybean variety and plants produced by such methods.