Soybean Variety A1035330 Breeding via Marker-Assisted Selection

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, leading to significant research costs and variability in resulting soybean varieties.

Innovation Solution

The development of the soybean variety A1035330, which incorporates specific breeding techniques like backcrossing and genetic transformation to introduce desired traits like herbicide resistance, disease resistance, and improved nutritional quality, while maintaining the morphological and physiological characteristics of the parent variety, using methods such as microprojectile bombardment and Agrobacterium-mediated transformation.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding techniques are used, then soybean varieties can be developed with desirable traits, but the process is time-consuming and unpredictable

Engineering Contradiction:
Improvepredictability of breeding outcomeVSAvoidbreeding development time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces conventional mechanical breeding methods (crossing, selection, and recombination of entire genomes) with biotechnological methods (genetic transformation, gene editing, and molecular marker-assisted selection). This substitution allows for precise introduction of specific traits without the time-consuming and unpredictable nature of traditional breeding, directly resolving the contradiction between reliability and time loss.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Reliability

If conventional breeding techniques are used, then soybean varieties can be developed, but research costs are significant

Engineering Contradiction:
Improvebreeding outcome consistencyVSAvoidresearch cost
Core Design Contradiction:
ReliabilityVSLoss of energy

Solution Approach 1:

The patent changes the fundamental parameters of the breeding process by using molecular markers to track specific genes and traits during breeding. This allows for early selection of desirable traits, reducing the number of generations needed and decreasing research costs while maintaining consistent, reliable breeding outcomes.

Inventive Principle:
Principle #35Parameter changes

3Productivity

If genetic transformation methods are used, then desired traits can be introduced efficiently, but the complexity of the breeding process increases

Engineering Contradiction:
Improvetrait introduction efficiencyVSAvoidbreeding process complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent uses molecular markers as intermediaries to simplify the genetic transformation process. Instead of directly introducing complex transgenic constructs, the markers serve as easily detectable indicators that guide the selection of successfully transformed plants, thereby increasing efficiency while managing process complexity.

Inventive Principle:
Principle #24Intermediary (Mediator)

4Stability of the object's composition

If backcrossing is used to introduce traits, then parental characteristics are maintained, but the breeding process becomes more complex

Engineering Contradiction:
Improveparental variety characteristicsVSAvoidbreeding procedure complexity
Core Design Contradiction:
Stability of the object's compositionVSDevice complexity

Solution Approach 1:

The patent replaces the complex mechanical process of repeated backcrossing with molecular marker-assisted selection. The markers allow for direct identification and selection of plants that have both the desired introduced trait and the parental variety's characteristic genome, eliminating the need for multiple backcrossing generations and simplifying the overall procedure.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS9179629B2Soybean variety A1035330
Publication Date: 2015.11.10 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated A1035330. Provided by the invention are the seeds, plants and derivatives of the soybean variety A1035330. Also provided by the invention are tissue cultures of the soybean variety A1035330 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety A1035330 with itself or another soybean variety and plants produced by such methods.