PCR Primers for Elite Soybean Event A5547-127 Detection
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Solution Overview
Problem
Current methods for identifying elite transgenic soybean event A5547-127 are complex, require extensive laboratory setups, and lack simplicity and speed, making it difficult to achieve unequivocal determination without expert interpretation.
Innovation Solution
Development of specific primers and probes that recognize the 5′ and/or 3′ flanking sequences of A5547-127 for use in PCR-based methods to amplify a unique DNA fragment, allowing for quick and simple identification of the event in biological samples.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional methods are used to identify elite transgenic soybean event A5547-127, then identification can be achieved, but the process becomes complex and requires extensive laboratory setups
Solution Approach 1:
The invention extracts and utilizes only the essential identifying features of the A5547-127 event - specifically the unique 5' and 3' flanking sequences - to create simplified PCR primers and probes. This extraction approach eliminates the need for complex laboratory setups while maintaining identification accuracy, as the method focuses solely on amplifying and detecting these specific flanking region sequences that are unique to the elite event.
2Reliability
If conventional identification methods are used, then elite event can be detected, but the process lacks speed and simplicity
Solution Approach 1:
The invention performs preliminary action by designing and preparing specific PCR primers and probes that are pre-configured to recognize and bind to the unique 5' and 3' flanking sequences of A5547-127. This preliminary preparation of event-specific molecular tools enables rapid amplification and detection in a single PCR step, significantly increasing identification speed while maintaining reliable detection through the specificity of the pre-designed primers and probes.
3Measurement precision
If standard identification approaches are used, then results can be obtained, but expert interpretation is required
Solution Approach 1:
The invention employs detection methods that produce distinct, easily distinguishable signals - such as specific band patterns on gels or fluorescent signals - that unequivocally indicate the presence of A5547-127. These visual or instrumental signals eliminate the need for expert interpretation, as the results provide clear, binary outcomes (present/absent) based on the specific amplification of flanking region sequences, making the method simple to operate and interpret.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid, accurate, and straightforward identification of A5547-127 in various biological samples, including plant material and products, without the need for extensive laboratory equipment or expert interpretation, ensuring reliable detection of the elite event.
Implementation Method 1
amplifying a sequence of a nucleic acid present in biological samples, using a polymerase chain reaction with at least two primers
Implementation Method 2
one of which recognizes the 5′ or 3′ flanking region of A5547-127, the other which recognizes a sequence within the foreign DNA
Data Source
AI summary
Tools are provided which allow rapid and unequivocal identification of elite event A5547-127 in biological samples.