Soybean Allergy Antigen Isolation for Trace IgE Detection
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Solution Overview
Problem
Conventional allergy testing agents for soybean allergies are inefficient due to the presence of varied allergen components, leading to inaccurate diagnosis and inability to detect IgE antibody reactions with allergen components present in trace amounts, which can affect symptom severity.
Innovation Solution
Identification of novel soybean allergens, such as Bowman-Birk type proteinase inhibitor D-II, Ribulose bisphosphate carboxylase large chain, Gamma-glutamyl hydrolase, and other specific proteins, which are used as antigens in diagnostic kits and compositions to accurately detect IgE antibody binding.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional allergy testing agents use crude antigen reagents containing multiple protein types, then the testing process is simple, but the diagnostic precision is insufficient and cannot detect trace allergen components
Solution Approach 1:
The patent extracts and isolates specific allergen components (such as Bowman-Birk inhibitor, Ribulose bisphosphate carboxylase large chain, Gamma-glutamyl hydrolase) from the complex crude antigen mixture. This extraction allows the testing agent to specifically detect trace allergen components while maintaining diagnostic precision, resolving the contradiction between simplicity and precision.
Solution Approach 2:
The patent segments the crude antigen into multiple distinct allergen components, each with specific identification numbers and characteristics. By dividing the complex mixture into separable components, the testing agent can accurately detect individual allergens even at trace levels, improving diagnostic precision without requiring overly complex testing procedures.
2Reliability
If conventional testing detects only abundant allergen components, then the testing method is straightforward, but it cannot identify sensitization to trace allergens that may cause severe reactions
Solution Approach 1:
The patent extracts specific allergen components that are present in trace amounts from the complex food matrix. By isolating these specific components (such as the identified soybean allergens with specific sequences), the testing agent can reliably detect sensitization to trace allergens that would otherwise be undetectable, improving diagnosis reliability.
Solution Approach 2:
The patent changes the detection parameters by using highly specific antigen reagents that can detect allergen components at very low concentrations. This parameter change enables the reliable detection of trace allergens that cause severe reactions, resolving the contradiction between reliability and detection difficulty.
3Measurement precision
If the antigen reagent contains varied amounts of different allergen components, then the reagent preparation is simple, but the diagnostic accuracy decreases due to cross-reactivity and inability to identify specific causative allergens
Solution Approach 1:
The patent extracts and isolates specific allergen components (such as Bowman-Birk inhibitor, Ribulose bisphosphate carboxylase large chain, Gamma-glutamyl hydrolase) from the crude antigen. This extraction process, while adding some manufacturing complexity, enables accurate identification of specific causative allergens and eliminates cross-reactivity issues, significantly improving allergen identification accuracy.
Solution Approach 2:
The patent segments the varied allergen components into individually identifiable units with specific sequences and characteristics. By dividing the complex mixture into separable, characterizable components, the reagent can accurately identify specific causative allergens, resolving the contradiction between manufacturing ease and identification accuracy.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enhances diagnostic precision by specifically identifying causative allergens, allowing for more accurate allergy diagnosis and targeted therapeutic interventions.
Implementation Method 1
IgE antibodies specific to particular antigens are produced. Physiological consequences caused by interaction between such IgE antibodies and such particular antigens elicit allergic reactions.
Data Source
AI summary
The present invention provides novel antigens of an allergy to soybean, methods and kits for diagnosing an allergy to soybean, pharmaceutical compositions comprising such an antigen, soybeans or processed products of soybean in which such an antigen is eliminated, and a tester for determining the presence or absence of a soybean antigen in an object of interest.