Soybean Variety D403301 Breeding via Transgenic Introgression

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, leading to significant research costs and variability in genetic outcomes.

Innovation Solution

The development of the soybean variety D403301, which combines improved traits through a specific breeding history and genetic transformation, including the introduction of a transgenic gene for glyphosate tolerance, and the use of advanced breeding techniques like backcrossing and genetic marker-assisted selection to introduce desired traits while maintaining the variety's morphological and physiological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional breeding techniques are used to develop soybean varieties, then genetic diversity can be explored, but the breeding process becomes time-consuming and unpredictable

Engineering Contradiction:
Improvegenetic diversityVSAvoidbreeding process time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-introducing specific transgenic genes (such as glyphosate tolerance genes) into selected parental lines before crossing. This allows breeders to start with genetically modified parents that already possess desired traits, eliminating the need to develop these traits through multiple generations of conventional breeding and selection, thus significantly reducing the overall breeding timeline while maintaining genetic diversity exploration.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses genetic transformation technology as an intermediary tool to directly introduce specific genes into soybean germplasm. This intermediary approach allows precise insertion of desired traits (herbicide tolerance, disease resistance) without relying on traditional cross-breeding and selection processes, thereby accelerating variety development while preserving the ability to explore genetic diversity through strategic parental selection.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Adaptability or versatility

If conventional breeding techniques are used, then natural genetic variation can be utilized, but research costs and variability in genetic outcomes increase

Engineering Contradiction:
Improvenatural genetic variationVSAvoidgenetic outcomes consistency
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The patent applies local quality by introducing specific transgenic traits into particular regions of the soybean genome through targeted genetic transformation. This allows precise control over which genes are modified while preserving the rest of the genome's natural variation. Breeders can select parental lines with desirable natural genetic variation and supplement them with specific transgenic traits, achieving both genetic diversity and predictable outcomes for key agronomic characteristics.

Inventive Principle:
Principle #3Local quality

Solution Approach 2:

The patent utilizes parameter changes by modifying specific genetic parameters (introducing transgenic genes for herbicide tolerance, disease resistance) while maintaining other genetic parameters through natural variation. This selective modification approach allows breeders to control key traits with high reliability while preserving genetic diversity in other areas, reducing overall variability in genetic outcomes for commercially important traits.

Inventive Principle:
Principle #35Parameter changes

3Productivity

If transgenic genes are introduced to improve specific traits, then breeding efficiency increases, but the complexity of the breeding program increases

Engineering Contradiction:
Improvebreeding efficiencyVSAvoidbreeding program complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent applies segmentation by dividing the breeding program into distinct modules: (1) selection of parental lines based on natural genetic variation, (2) introduction of specific transgenic traits through genetic transformation, and (3) conventional crossing and selection procedures. This segmentation allows each component to be optimized independently, improving overall breeding efficiency while managing complexity through structured organization of breeding activities.

Inventive Principle:
Principle #1Segmentation

Data Source

PatentUS9338974B2Soybean variety D403301
Publication Date: 2016.05.17 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated D403301. Provided by the invention are the seeds, plants and derivatives of the soybean variety D403301. Also provided by the invention are tissue cultures of the soybean variety D403301 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety D403301 with itself or another soybean variety and plants produced by such methods.