Soybean D4258962 Breeding via Molecular Marker Selection

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Solution Overview

Problem

The development of new soybean varieties is a time-consuming and unpredictable process due to the complexity of combining desirable traits such as higher seed yield, disease resistance, and environmental adaptability, with conventional breeding methods lacking control at the DNA level and resulting in large research expenditures.

Innovation Solution

The soybean variety D4258962 is developed through a specific breeding program involving crossing and selection methods, including backcrossing and genetic transformation, to introduce desired traits like herbicide resistance, disease resistance, and improved nutritional quality, while maintaining the elite genetic background for further variety development.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If conventional breeding methods are used to combine desirable traits, then trait combination is achieved, but the process is time-consuming and unpredictable

Engineering Contradiction:
Improvebreeding efficiencyVSAvoidbreeding cycle duration
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

The patent applies parameter changes by transitioning from conventional phenotypic selection to molecular marker-assisted selection. This involves changing the selection parameter from observable traits to DNA-based markers, enabling precise identification and selection of plants with desired traits at the genetic level, thereby reducing breeding time and increasing predictability.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent replaces the mechanical/phenotypic selection system with a molecular biological system. Instead of relying on physical observation and manual selection processes, the invention uses DNA markers and molecular techniques to identify and select plants, substituting the conventional breeding mechanics with modern molecular tools.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Productivity

If conventional breeding methods are used, then variety development is achieved, but research expenditures are large

Engineering Contradiction:
Improvevariety development outputVSAvoidresearch expenditure
Core Design Contradiction:
ProductivityVSLoss of energy

Solution Approach 1:

The patent replaces expensive conventional breeding operations with cost-effective molecular marker analysis. By using DNA markers to predict trait inheritance, the system reduces the need for extensive field testing, multiple generations of breeding, and large-scale phenotypic evaluation, thereby significantly reducing research expenditures while maintaining variety development output.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent uses molecular markers as copies or proxies for actual trait expression. Instead of directly observing and selecting for complex traits that require extensive resource investment, the system uses easily detectable DNA markers that correlate with desired traits, providing a cost-effective surrogate for trait selection.

Inventive Principle:
Principle #26Copying

3Adaptability or versatility

If conventional breeding methods are used, then trait combination is achieved, but control at DNA level is lacking

Engineering Contradiction:
Improvetrait combination capabilityVSAvoidgenetic control precision
Core Design Contradiction:
Adaptability or versatilityVSManufacturing precision

Solution Approach 1:

The patent substitutes phenotypic selection with molecular-level control. By using DNA markers and molecular biology techniques, the system achieves precise control at the genetic level, enabling accurate prediction and manipulation of trait inheritance patterns that cannot be achieved through conventional observational selection methods.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent introduces molecular markers as intermediary elements between the breeder and the genetic traits. These markers serve as mediators that provide direct information about gene presence and inheritance patterns, enabling precise genetic control without requiring direct manipulation of the complex trait expressions themselves.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS7521605B2Soybean variety D4258962
Publication Date: 2009.04.21 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated D4258962. Provided by the invention are the seeds, plants and derivatives of the soybean variety D4258962. Also provided by the invention are tissue cultures of the soybean variety D4258962 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety D4258962 with itself or another soybean variety and plants produced by such methods.