Soybean DLL1141 Herbicide Resistance via Molecular Markers

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Solution Overview

Problem

Soybean breeding faces challenges in predicting phenotypic expression due to genetic complexity and environmental factors, making it difficult to replicate stable, high-yielding varieties, and limiting breeders' ability to produce identical varieties using the same parents and methods.

Innovation Solution

Development of the soybean variety DLL1141, which includes a transgene conferring herbicide resistance and other desirable traits, allowing for self-pollination, backcrossing, and hybrid production, along with methods like microprojectile-mediated delivery and Agrobacterium-mediated transformation to introduce genes for enhanced characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional breeding methods are used to develop soybean varieties, then variety diversity can be achieved, but phenotypic expression cannot be predicted due to genetic complexity and environmental factors

Engineering Contradiction:
Improvevariety diversityVSAvoidphenotypic expression prediction
Core Design Contradiction:
Adaptability or versatilityVSMeasurement precision

Solution Approach 1:

The patent replaces conventional phenotypic selection methods with molecular marker-assisted selection. By using DNA markers linked to desirable traits, breeders can directly assess genotype without relying on phenotypic expression, thereby eliminating the unpredictability caused by environmental factors while maintaining variety diversity.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent introduces molecular markers as an intermediary between genotype and phenotype. These markers serve as reliable indicators of genetic composition, allowing breeders to predict and select for desirable traits with high precision while maintaining the diversity achieved through conventional breeding.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Stability of the object's composition

If the same parents and breeding methods are used, then breeding consistency is expected, but identical varieties cannot be produced due to genetic complexity

Engineering Contradiction:
Improvebreeding consistencyVSAvoidvariety replication
Core Design Contradiction:
Stability of the object's compositionVSManufacturing precision

Solution Approach 1:

The patent replaces phenotypic observation with molecular marker analysis to ensure breeding consistency. By selecting plants based on their genetic profile rather than phenotypic expression, the same breeding parents and methods can reliably produce identical varieties with the same genetic composition.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent enables precise replication of varieties by using molecular markers to copy the exact genetic composition of successful varieties. Breeders can identify and propagate plants with identical marker profiles, ensuring variety replication regardless of environmental variations.

Inventive Principle:
Principle #26Copying

3Measurement precision

If molecular marker-assisted selection is implemented, then phenotypic prediction accuracy is improved, but breeding process complexity increases

Engineering Contradiction:
Improvephenotypic expression predictionVSAvoidbreeding process complexity
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The patent performs molecular marker analysis on young plants or seedlings rather than waiting for phenotypic expression. This preliminary genetic assessment allows early selection of desirable traits, reducing the time and resources needed for field testing and simplifying the overall breeding process.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent shifts the selection parameter from phenotypic traits (which are environmentally influenced) to genotypic markers (which are stable and predictable). This parameter change simplifies the breeding process by providing clear, unambiguous selection criteria that do not require complex environmental control or interpretation.

Inventive Principle:
Principle #35Parameter changes

4Adaptability or versatility

If genetic engineering techniques are used to introduce transgenes, then desirable traits such as herbicide resistance can be conferred, but regulatory and safety assessment requirements increase

Engineering Contradiction:
Improvetrait enhancementVSAvoidregulatory complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent uses molecular markers as intermediaries to track and verify the presence and expression of transgenes. This marker-assisted approach provides precise documentation of genetic modification, facilitating regulatory assessment and safety verification while enabling the introduction of desirable traits like herbicide resistance.

Inventive Principle:
Principle #24Intermediary (Mediator)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Soybean variety DLL1141 demonstrates stability and superior characteristics, enabling the production of hybrid seeds with improved traits such as herbicide resistance, disease resistance, and nutritional quality, facilitating consistent breeding and genetic modification for enhanced agronomic performance.

Implementation Method 1

which includes a transgene conferring herbicide resistance

Methodology Applied
Scientific EffectHerbicide resistance:

Implementation Method 2

methods like microprojectile-mediated delivery and Agrobacterium-mediated transformation to introduce genes

Methodology Applied
Scientific EffectMicroprojectile-mediated delivery:

Implementation Method 3

methods like microprojectile-mediated delivery and Agrobacterium-mediated transformation to introduce genes

Methodology Applied
Scientific EffectAgrobacterium-mediated transformation:

Data Source

PatentUS9006533B2Soybean variety DLL1141
Publication Date: 2015.04.14 BASF AGRICULTURAL SOLUTIONS US LLC

AI summary

The soybean variety DLL1141 is disclosed. The invention relates to seeds, plants, plant cells, plant tissue, harvested products and soybean lint as well as to hybrid soybean plants and seeds obtained by repeatedly crossing plants of variety DLL1141 with other plants. The invention also relates to plants and varieties produced by the method of essential derivation from plants of DLL1141 and to plants of DLL1141 reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from DLL1141.