Soybean Variety ELL0254 Breeding for Predictable Phenotypes

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Solution Overview

Problem

Soybean breeding faces challenges in predicting phenotypic expression due to genetic complexity and environmental factors, making it difficult to replicate stable, high-yielding varieties, and existing methods limit the ability to breed the same variety twice using the same parents and methodology.

Innovation Solution

Development of the soybean variety ELL0254, which includes methods for crossing, mutagenesis, and transformation to produce hybrid seeds and plants with superior characteristics, such as herbicide resistance, disease resistance, and enhanced nutritional quality, using techniques like microprojectile-mediated delivery and Agrobacterium-mediated transformation.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to develop soybean varieties, then variety diversity can be achieved, but phenotypic expression cannot be predicted due to genetic complexity and environmental factors

Engineering Contradiction:
Improvevariety diversityVSAvoidphenotypic expression prediction
Core Design Contradiction:
Adaptability or versatilityVSMeasurement precision

Solution Approach 1:

The patent creates essentially derived varieties by copying the genetic material of the original soybean variety through controlled breeding methods. These derived varieties replicate the desirable traits of the parent variety while allowing for systematic improvement, thus achieving predictability in phenotypic expression while maintaining variety diversity

Inventive Principle:
Principle #26Copying

Solution Approach 2:

The patent employs preliminary selection of parent varieties with known and characterized genetic traits before initiating the breeding program. By pre-characterizing the parental lines and selecting for specific genetic markers, the breeder can predict the phenotypic expression of offspring more accurately, resolving the uncertainty caused by genetic complexity

Inventive Principle:
Principle #10Preliminary action

2Productivity

If the same parents and methodology are used in breeding, then breeding efficiency should improve, but the same variety cannot be bred twice due to genetic variability

Engineering Contradiction:
Improvebreeding efficiencyVSAvoidvariety reproducibility
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent implements a feedback mechanism by systematically evaluating and characterizing each bred variety against the parent variety's traits. Through controlled backcrossing and selection, the breeder monitors genetic composition and adjusts breeding strategies to maintain desired traits, enabling reproduction of successful varieties while managing genetic variability

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The patent modifies specific genetic parameters through controlled breeding operations such as backcrossing, selfing, and selection. By changing the proportion of parental genetics in successive generations (e.g., maintaining 93.75% of parent variety genetics in F5 progeny), the breeder can reproduce successful varieties with predictable outcomes while allowing for necessary genetic adjustments

Inventive Principle:
Principle #35Parameter changes

3Reliability

If multiple breeding operations are performed to improve variety traits, then variety quality can be enhanced, but the complexity of breeding operations increases

Engineering Contradiction:
Improvevariety qualityVSAvoidbreeding operation complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent divides the breeding program into distinct, manageable operations: initial crossbreeding to create F1 hybrids, followed by systematic backcrossing operations (BC1, BC2, etc.), and final selection phases. Each operation has specific objectives and selection criteria, making the complex process of improving variety quality more controllable and repeatable

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent employs dynamic breeding strategies where the number and type of breeding operations are adjusted based on the progress and results of previous generations. The breeder can intensify selection pressure or modify crossbreeding schemes in response to observed trait expression, optimizing the balance between variety quality improvement and operational complexity

Inventive Principle:
Principle #15Dynamics

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Soybean variety ELL0254 demonstrates uniformity and stability, offering superior characteristics like herbicide resistance and disease resistance, and allows for the production of hybrid seeds and plants with improved agronomic traits through controlled breeding and genetic engineering methods.

Implementation Method 1

using techniques like microprojectile-mediated delivery and Agrobacterium-mediated transformation

Methodology Applied
Scientific EffectMicroprojectile-mediated delivery:

Implementation Method 2

using techniques like microprojectile-mediated delivery and Agrobacterium-mediated transformation

Methodology Applied
Scientific EffectAgrobacterium-mediated transformation:

Data Source

PatentUS9699984B1Soybean variety ELL0254
Publication Date: 2017.07.11 BASF AGRICULTURAL SOLUTIONS US LLC

AI summary

The soybean variety ELL0254 is disclosed. The invention relates to seeds, plants, plant cells, plant tissue, harvested products and soybean lint as well as to hybrid soybean plants and seeds obtained by repeatedly crossing plants of variety ELL0254 with other plants. The invention also relates to plants and varieties produced by the method of essential derivation from plants of ELL0254 and to plants of ELL0254 reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from ELL0254.