Transgenic Soybean Event 127 Herbicide Tolerance Detection
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Solution Overview
Problem
There is a need for soybean plants that are tolerant to herbicides other than glyphosate to manage weeds, as the increased use of glyphosate-tolerant soybeans has led to the emergence of glyphosate-tolerant weeds, and existing methods for detecting transgenic events are complex and time-consuming, requiring extensive laboratory setups.
Innovation Solution
Development of transgenic soybean plants with a heterologous nucleic acid molecule encoding a mutant acetohydroxyacid synthase large subunit (AHASL) protein, which confers resistance to AHAS-inhibiting herbicides, along with methods for identifying and detecting these plants using specific nucleic acid primers and probes, allowing for efficient weed control and crop safety.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If transgenic soybean plants are developed to provide herbicide tolerance, then weed control capability is improved, but the complexity of detecting and identifying transgenic events increases
Solution Approach 1:
The patent applies preliminary action by pre-characterizing the transgenic event 127 and developing specific detection methods (PCR primers, probes, and protocols) before commercial deployment. This allows for straightforward identification of the transgene in soybean plants and their progeny, eliminating the need for complex phenotypic characterization and reducing detection complexity while maintaining herbicide tolerance capability
2Reliability
If extensive genetic characterization and field trials are conducted, then reliability of transgene introgression is improved, but time required for event identification increases
Solution Approach 1:
The patent replaces complex mechanical and phenotypic characterization systems with molecular detection systems (PCR-based methods). Instead of conducting extensive field trials and phenotypic evaluations to identify transgenic events, the invention uses specific PCR primers and probes that directly detect the presence and characteristics of event 127 DNA, dramatically reducing the time required while maintaining reliability through molecular specificity
Solution Approach 2:
The patent uses DNA copying and amplification (PCR) to detect the transgene. By amplifying specific DNA sequences from the transgenic event 127 using predetermined primers and probes, the method creates detectable copies of the transgene presence, allowing rapid identification without time-consuming phenotypic expression analysis or extensive field trial data collection
3Measurement precision
If specific detection methods are developed for event 127, then measurement precision of transgenic events is improved, but ease of operation decreases
Solution Approach 1:
The patent achieves universality by developing a multi-functional detection system that uses the same PCR-based platform for multiple purposes: detecting the presence of event 127, characterizing the transgene integration, and monitoring progeny inheritance. This unified approach maintains high measurement precision through specific primers and probes while improving ease of operation by eliminating the need for multiple different detection methodologies
Data Source
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AI summary
Compositions and methods related to transgenic AHAS-inhibiting herbicide resistant soybean plants are provided. Event 127 soybean plants having a mutated AHAS coding sequence which imparts tolerance to an AHAS-inhibiting herbicide are provided. The event 127 soybean plants having the event 127 nucleic acid molecule at the identified chromosomal location may comprise genomic/transgene junctions having at least the nucleic acid sequence of SEQ ID NO: 5 and/or 6. The characterization of the genomic insertion site of the event 127 provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event 127 soybean plants are provided.