Soybean Extract Production via Yeast Fermentation
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Solution Overview
Problem
Current techniques for obtaining soybean extracts are at an exploratory level, and there is a need for a simple and industrially exploitable method to produce active extracts with antioxidant, anti-inflammatory, and anti-elastase properties.
Innovation Solution
A process involving soy juice extraction, ultrafiltration, concentration by reverse osmosis or evaporation, hydrolysis using baker's yeast (e.g., Saccharomyces cerevisiae) to break down sugars, and subsequent dehydration and desalting to obtain a rich extract of manninotriose and melibiose, which are then combined with edible silica to enhance stability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If current exploratory techniques are used to obtain soybean extracts, then research flexibility is maintained, but industrial scalability and simplicity are insufficient
Solution Approach 1:
The extraction process is divided into distinct sequential stages: extraction of soy juice, ultrafiltration to separate permeate, concentration of permeate, and controlled fermentation hydrolysis. This segmentation allows each step to be optimized independently for both simplicity and industrial scalability, transforming the exploratory technique into an industrially viable process.
Solution Approach 2:
The invention specifies precise parameter ranges including ultrafiltration membrane cut-off (5,000-50,000 daltons), fermentation temperature (20-40°C), and pH control (4.0-6.0) to optimize both the simplicity of operation and productivity. By defining these parameters, the process transitions from exploratory to industrially scalable while maintaining ease of manufacture.
2Manufacturing precision
If baker's yeast fermentation is used for hydrolysis, then selectivity for manninotriose and melibiose production is improved, but process time is extended
Solution Approach 1:
The fermentation process is conducted as a controlled periodic operation with defined stages: initial adaptation phase, active hydrolysis phase, and termination phase. By controlling the duration of each phase and monitoring sugar consumption patterns, the process achieves high compositional purity of manninotriose and melibiose while limiting excessive fermentation time that would lead to complete sugar depletion.
Solution Approach 2:
The process utilizes feedback control by monitoring the consumption of raffinose and stacchyose during fermentation and adjusting conditions to terminate the process at the optimal point when manninotriose and melibiose concentrations are maximized. This feedback mechanism ensures high manufacturing precision while minimizing unnecessary fermentation time.
3Quantity of substance
If multiple fermentation organisms are used for sugar hydrolysis, then complete sugar breakdown is achieved, but selectivity for specific oligosaccharides is lost
Solution Approach 1:
The invention extracts or selects only the essential fermentative properties of baker's yeast (invertase activity) while deliberately excluding organisms with melibiase activity. By using exclusively baker's yeast, the process achieves both sufficient sugar hydrolysis and high selectivity for manninotriose and melibiose production, as these yeasts lack the enzymes that would further degrade these valuable oligosaccharides.
Solution Approach 2:
The fermentation system is designed with local quality by assigning specific enzymatic capabilities to the selected organism. Baker's yeast provides invertase activity for hydrolyzing the β1-2 glucose-fructose bond in raffinose and stacchyose, while deliberately lacking melibiase activity. This localized enzymatic property ensures selective production of the desired oligosaccharides without complete sugar breakdown.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process effectively produces a nutraceutical composition with demonstrated antioxidant, anti-inflammatory, and anti-elastase properties, significantly reducing reactive oxygen species, myeloperoxidase, and elastase activities, while inhibiting inflammatory cytokines.
Implementation Method 1
a step for obtaining a permeate by ultrafiltration of said soy juice, the permeate thus obtained being essentially free of lipids and proteins
Implementation Method 2
a step for concentrating said permeate, advantageously by reverse osmosis
Implementation Method 3
stage during which an enzymatic hydrolysis of the sugars resulting from the invertase activity of said baker's yeast is carried out
Implementation Method 4
at least one stage of hydrolysis of said permeate by the fermentation route carried out using at least one baker's yeast
Data Source
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AI summary
The invention relates to a method for production of at least one active extract rich in manninotriose and melibiose from soya beans comprising at least one soya juice extraction step, a step for production of a permeate by ultrafiltration of the soya juice, said permeate being essentially free of lipids and proteins and at least one hydrolysis step of said permeate by fermentation carried out by means of at least one baker's yeast in the absence of any lactic fermentation and during this step an enzymatic hydrolysis of the sugars produced by the invertase activity of said baker's yeast is carried out to give said active extract.