Soybean GLL0059 Herbicide Resistance via Marker-Assisted Selection

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Solution Overview

Problem

Soybean breeding faces challenges in predicting phenotypic expression due to genetic complexity and environmental factors, making it difficult to reproduce stable, high-yielding varieties consistently.

Innovation Solution

Development of the soybean variety GLL0059, which includes a transgene conferring herbicide resistance and other desirable traits, along with methods for producing hybrid seeds and plants through selfing, backcrosses, and genetic transformation, enabling the creation of regenerable cells for tissue culture and introduction of genes for resistance, nutritional enhancement, and industrial usage.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional breeding methods are used to develop soybean varieties, then variety diversity can be achieved, but phenotypic expression cannot be predicted due to genetic complexity and environmental factors

Engineering Contradiction:
Improvevariety diversityVSAvoidphenotypic prediction accuracy
Core Design Contradiction:
Adaptability or versatilityVSMeasurement precision

Solution Approach 1:

The patent replaces conventional phenotypic selection methods with molecular marker-based genotypic selection. By using DNA markers linked to desirable traits, breeders can directly assess genotype without relying on phenotypic expression, thereby eliminating the unpredictability caused by environmental factors and genetic complexity while maintaining variety diversity.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Stability of the object's composition

If multiple breeding methods are applied to create stable varieties, then variety stability can be improved, but breeding time and complexity increase

Engineering Contradiction:
Improvevariety stabilityVSAvoidbreeding time
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The patent applies preliminary action by using molecular markers to identify and select plants with desired genotypes early in the breeding process, before phenotypic expression occurs. This allows breeders to make informed selection decisions at the seedling stage rather than waiting for mature plant evaluation, significantly reducing the time required to develop stable varieties while maintaining genetic stability through marker-assisted selection.

Inventive Principle:
Principle #10Preliminary action

3Ease of manufacture

If conventional breeding selection is used, then desirable traits can be selected, but the breeder can only work with phenotype and not directly with genotype

Engineering Contradiction:
Improvetrait selection capabilityVSAvoidgenotype information accessibility
Core Design Contradiction:
Ease of manufactureVSLoss of information

Solution Approach 1:

The patent replaces phenotypic observation with molecular marker analysis, enabling direct genotypic assessment. By using DNA-based markers that are tightly linked to target genes, breeders can directly evaluate genotype information without relying on phenotypic expression, thereby recovering the lost genotype information and improving trait selection accuracy and ease.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS9538717B1Soybean variety GLL0059
Publication Date: 2017.01.10 BASF AGRICULTURAL SOLUTIONS US LLC

AI summary

The soybean variety GLL0059 is disclosed. The invention relates to seeds, plants, plant cells, plant tissue, harvested products and soybean lint as well as to hybrid soybean plants and seeds obtained by repeatedly crossing plants of variety GLL0059 with other plants. The invention also relates to plants and varieties produced by the method of essential derivation from plants of GLL0059 and to plants of GLL0059 reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from GLL0059.