Soybean HLL0771 Breeding via Molecular Marker Selection

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Solution Overview

Problem

Soybean breeding faces challenges in predicting phenotypic expression due to genetic complexity and environmental factors, making it difficult to replicate stable, high-yielding varieties, and existing methods limit the ability to breed the same variety twice using the same parents and methodology.

Innovation Solution

The development of soybean variety HLL0771, which includes a transgene conferring LibertyLink herbicide resistance, and methods for producing hybrid seeds and plants with superior characteristics through selfing, backcrosses, and genetic transformation using expression vectors and tissue culture techniques.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to develop soybean varieties, then variety diversity can be achieved, but the ability to predict and replicate phenotypic expression is poor due to genetic complexity and environmental factors

Engineering Contradiction:
Improvevariety diversityVSAvoidphenotypic expression prediction
Core Design Contradiction:
Adaptability or versatilityVSMeasurement precision

Solution Approach 1:

The patent replaces traditional phenotypic selection methods with molecular marker-assisted selection. Instead of relying on visual phenotypic assessment which is imprecise due to environmental influences, the invention uses DNA markers to directly identify and select plants with desired genotypes, enabling accurate prediction and replication of trait expression across environments

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent introduces molecular markers as an intermediary between genotype and phenotype. These markers serve as reliable indicators that allow breeders to indirectly select for desired traits with high precision, overcoming the limitation of direct phenotypic observation while maintaining genetic diversity

Inventive Principle:
Principle #24Intermediary (Mediator)

2Stability of the object's composition

If the same breeding methodology is applied repeatedly with the same parents, then consistency in breeding process is achieved, but the ability to breed the same variety twice is limited due to unpredictable phenotypic outcomes

Engineering Contradiction:
Improvebreeding process consistencyVSAvoidvariety replication
Core Design Contradiction:
Stability of the object's compositionVSReliability

Solution Approach 1:

The patent implements feedback through molecular marker analysis at multiple stages of the breeding process. By continuously monitoring genotype-marker associations and using this information to guide subsequent selection and crossing decisions, the system ensures both process consistency and reliable variety replication

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The patent performs preliminary molecular marker analysis on parent plants before crossing to identify and select parents with complementary desirable traits. This preliminary genotypic assessment ensures that subsequent breeding cycles can reliably produce the same variety with consistent trait expression

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS10555468B1Soybean variety HLL0771
Publication Date: 2020.02.11 BASF AGRICULTURAL SOLUTIONS US LLC

AI summary

The soybean variety HLL0771 is disclosed. The invention relates to seeds, plants, plant cells, plant tissue, harvested products and soybean lint as well as to hybrid soybean plants and seeds obtained by repeatedly crossing plants of variety HLL0771 with other plants. The invention also relates to plants and varieties produced by the method of essential derivation from plants of HLL0771 and to plants of HLL0771 reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from HLL0771.