Soybean Transgenic Event IND-ØØ41Ø-5 Drought Tolerance
Solution Overview
Problem
Current methods for developing transgenic soybean plants with desirable traits like drought tolerance are inefficient, requiring extensive characterization and testing, and existing patents do not provide commercially viable soybean plants with combined abiotic stress resistance and specific trait preservation.
Innovation Solution
The development of soybean event IND-ØØ41Ø-5, which includes a single copy of the bar marker gene and the HaHB4 gene conferring abiotic stress tolerance, integrated into the soybean genome using specific promoter sequences and transformation methods, resulting in plants resistant to multiple abiotic stresses and allowing for further trait stacking.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If transgenic events are developed through extensive characterization and testing, then desired traits like drought tolerance are achieved, but the process becomes time-consuming and resource-intensive
Solution Approach 1:
The patent applies preliminary action by pre-characterizing multiple transgenic events in the laboratory phase before field testing. Extensive molecular and phenotypic characterization is performed upfront to identify events with desired traits, thereby reducing the time required during actual field trials and accelerating the overall development process.
Solution Approach 2:
The development process is segmented into distinct phases: laboratory development with molecular characterization, greenhouse testing, and field trials. This segmentation allows parallel processing of multiple events in the laboratory phase, reducing the overall time required while maintaining rigorous characterization standards for reliability.
2Adaptability or versatility
If multiple transgenic events are analyzed under various environmental conditions, then the most suitable event is selected, but the complexity of the selection process increases
Solution Approach 1:
The patent applies local quality by evaluating transgenic events under specific local environmental conditions at multiple field locations. Different events are assessed for their performance in particular environmental contexts, allowing selection of events with optimal local adaptation while managing selection complexity through focused, location-specific trials.
Solution Approach 2:
The patent employs universal selection criteria that can be applied across multiple environmental conditions and locations. The same molecular and phenotypic characterization protocols are used consistently across different environments, providing a universal framework for event selection that manages complexity through standardization while assessing adaptability.
3Measurement precision
If transgenic events are extensively characterized molecularly and phenotypically, then events with desired traits are identified, but the cost and resources required increase
Solution Approach 1:
The patent applies preliminary action by performing extensive molecular and phenotypic characterization in the laboratory phase before field testing. Multiple events are screened upfront using precise measurement techniques to identify those with desired traits, thereby reducing the number of events requiring resource-intensive field trials while maintaining high measurement precision for trait identification.
Solution Approach 2:
The patent extracts and focuses resources on characterizing only those transgenic events that show promise in preliminary laboratory screening. By taking out the most promising events from the larger pool and concentrating resources on their detailed characterization, the patent maintains measurement precision while reducing overall resource consumption.
Data Source
AI summary
The invention relates to the fields of plant production, plant breeding and agriculture. More specifically, it relates to soybean transgenic event IND-ØØ41Ø-5, which expresses the gene HaHB4 that confers drought tolerance without affecting other agricultural capabilities. The application also discloses related nucleotide sequences, plants, parts of plants, seeds, cells, agricultural products, and detection and production methods.