Soybean Lr67 Allele Mutations for Rust Resistance
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Solution Overview
Problem
Current methods fail to provide significant resistance against fungal pathogens, particularly rust fungi like Phakopsora pachyrhizi, in soybean plants, despite efforts to introduce the wheat Lr67res gene, which did not yield statistically significant results.
Innovation Solution
Introduction of specific mutations in the soybean Lr67 gene, such as G145R, G145K, and 1389L, to create an Lr67 allele that enhances resistance by reducing susceptibility to biotrophic and heminecrotrophic fungi, specifically through substitutions at positions G145 and 1389, which are incorporated into plants via transgenic expression or mutagenesis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If the wheat Lr67res gene is introduced into soybean plants, then resistance against fungal pathogens is expected to improve, but the results are not statistically significant
Solution Approach 1:
The patent applies parameter changes by introducing specific mutations (G145R, G145K, 1389L) in the soybean Lr67 gene to create an Lr67 allele that significantly enhances resistance against biotrophic and heminecrotrophic fungi, resolving the ineffectiveness of the original wheat Lr67res gene introduction
2Productivity
If monocultures are used for soybean production, then productivity is improved, but susceptibility to epidemic spreading of diseases increases
Solution Approach 1:
The patent applies preliminary anti-action by introducing the modified Lr67 allele into soybean plants before fungal infection occurs, providing pre-established resistance that prevents epidemic spreading while maintaining monoculture productivity
3Reliability
If pesticides are used to control fungal pathogens, then disease control is improved, but environmental harm and pesticide resistance increase
Solution Approach 1:
The patent replaces the mechanical/chemical system of pesticide application with a biological system by introducing the modified Lr67 allele that provides inherent fungal resistance through genetic modification, eliminating the need for chemical pesticides
Data Source
AI summary
The present invention relates to an Lr67 allele differing from a soybean Lr67 wild type gene as defined herein. The allele unexpectedly conveys, intensifies or stabilizes fungal pathogen tolerance or resistance to a plant, seed, cell, or other plant part, most preferably against rusts of genus Phakopsora in leguminous plants or parts thereof. The invention in particular provides cells, expression constructs and plants and parts thereof, for example seed, comprising the Lr67 allele, and products obtainable or obtained therefrom. The invention also provides ensembles or populations of plants or accumulations of seed. Furthermore, the invention provides uses and methods to make use of the Lr67 allele of the invention and to realize the advantages conferred thereby. In particular, the invention provides methods for creating a cell, plant or part thereof comprising the Lr67 allele, and methods for producing a plant population having resistance against a fungal pathogen. Further methods according to the invention relate to the reduction or abolition of susceptibility of a plant or part thereof to infections by a fungal pathogen, for assaying the susceptibility to fungal pathogen infection and for preparation of feed or food products. The Lr67 allele of the invention can be incorporated into the plant transgenically or via man-directed mutagenesis.