Soybean Breeding Acceleration via Marker-Assisted Selection

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Solution Overview

Problem

The development of new soybean cultivars is a time-consuming process that requires precise planning and efficient resource use, aiming to combine desirable traits such as higher seed yield, disease resistance, drought tolerance, and improved fatty acid profile, while existing methods are limited in achieving these goals efficiently.

Innovation Solution

The introduction of soybean cultivar 06210302, which involves crossing with itself or other cultivars, and methods like mutagenesis or transformation to introduce transgenic or mutant traits, along with tissue culture techniques for regenerating plants with specific characteristics, such as herbicide resistance and improved agronomic traits.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional soybean breeding methods are used to combine desirable traits, then genetic diversity and trait combination are improved, but the breeding process becomes extremely time-consuming and resource-intensive

Engineering Contradiction:
Improvetrait combinationVSAvoidbreeding process duration
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-characterizing parental lines for specific desirable traits (herbicide tolerance, disease resistance, fatty acid profile) before crossing. This allows breeders to select parents with known trait combinations, reducing the time needed for screening and evaluation in later generations while ensuring the desired traits are present in the progeny.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses molecular markers to copy or track specific trait-associated DNA sequences through generations. By using marker-assisted selection, breeders can identify and select plants carrying desired traits without phenotypic expression, significantly accelerating the breeding process while maintaining trait combination accuracy.

Inventive Principle:
Principle #26Copying

2Stability of the object's composition

If multiple crossing and selection cycles are performed to achieve stable cultivars, then genetic uniformity and stability are improved, but productivity and resource efficiency deteriorate

Engineering Contradiction:
Improvegenetic uniformityVSAvoidbreeding output per time
Core Design Contradiction:
Stability of the object's compositionVSProductivity

Solution Approach 1:

The patent replaces mechanical/phenotypic selection methods with molecular marker-based selection. Instead of visually screening thousands of plants for desired traits through multiple generations, breeders use DNA markers to identify and select plants with desired genetic compositions in the early generations, reducing the number of cycles needed to achieve genetic uniformity while increasing breeding productivity.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent changes the selection parameter from phenotypic expression to genotypic identification. By using molecular markers to detect trait-associated DNA sequences, the selection process occurs at the molecular level rather than waiting for phenotypic expression, thereby reducing the time and cycles required to achieve stable, uniform cultivars while maintaining high breeding output.

Inventive Principle:
Principle #35Parameter changes

3Productivity

If focus is placed on improving yield and compositional traits, then agronomic quality is improved, but resistance to diseases and environmental stresses may be compromised

Engineering Contradiction:
Improveseed yieldVSAvoiddisease resistance
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent merges multiple trait selection criteria into a single integrated breeding program. By simultaneously selecting for yield, compositional traits (fatty acid profile), and resistance traits (disease and stress tolerance) using marker-assisted selection, the program maintains balanced improvement across all parameters without compromising any single trait, achieving high productivity while ensuring reliability through combined trait selection.

Inventive Principle:
Principle #5Merging (Combining)

Data Source

PatentUS11771039B2Soybean cultivar 06210302
Publication Date: 2023.10.03 M S TECH LLC

AI summary

A soybean cultivar designated 06210302 is disclosed. The invention relates to the seeds of soybean cultivar 06210302, to the plants of soybean cultivar 06210302, to the plant parts of soybean cultivar 06210302, and to methods for producing progeny of soybean cultivar 06210302. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 06210302. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 06210302, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 06210302 with another soybean cultivar.