Soybean Seed Sucrosyl-Oligosaccharide Measurement via Near-Infrared Spectroscopy
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current analytical methods for measuring sucrosyl-oligosaccharides like stachyose and raffinose in soybean seeds are destructive, time-consuming, and require large sample sizes, limiting their efficiency and accuracy in assessing seed composition.
Innovation Solution
Non-destructive near-infrared spectroscopy methods are employed to measure sucrosyl-oligosaccharides in soybean seeds by directing near-infrared light onto the seeds, allowing for accurate determination of their concentration using imaging devices, enabling precise analysis without damaging the seeds.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If destructive analytical methods are used to measure sucrosyl-oligosaccharides in soybean seeds, then measurement accuracy can be achieved, but the seeds are damaged and cannot be used for further analysis or processing
Solution Approach 1:
The patent replaces destructive mechanical/chemical analysis methods with non-destructive near-infrared spectroscopy. The NIRS system uses electromagnetic radiation to interact with molecular bonds in the seed, allowing measurement of sucrosyl-oligosaccharide content without physically damaging the seed structure, thus resolving the contradiction between measurement accuracy and seed preservation
Solution Approach 2:
The patent changes the physical state and properties of the measurement process by using near-infrared light interaction instead of destructive chemical extraction. By utilizing the absorption characteristics of specific chemical bonds at near-infrared wavelengths, the system achieves accurate measurement while maintaining the seed in its original state for potential reuse
2Measurement precision
If standard reference analytical methods are used to measure sucrosyl-oligosaccharides, then accurate results can be obtained, but the process is time-consuming and requires large sample sizes
Solution Approach 1:
The patent replaces time-consuming mechanical extraction and chemical analysis procedures with rapid optical measurement using near-infrared spectroscopy. The NIRS system provides real-time or near-real-time measurements without the lengthy sample preparation, extraction, and analysis steps required by traditional methods, dramatically improving analysis throughput and efficiency
Solution Approach 2:
The patent creates a spectral fingerprint copy of the seed's chemical composition through near-infrared absorption patterns. By measuring the characteristic absorption spectra of sucrosyl-oligosaccharides without physically extracting them, the system obtains accurate compositional information rapidly, eliminating the time-consuming nature of reference analytical methods while maintaining measurement precision
3Measurement precision
If large sample sizes are required for analysis, then representative measurements can be obtained, but the process becomes less efficient and more resource-intensive
Solution Approach 1:
The patent replaces bulk sample analysis with targeted molecular detection using near-infrared spectroscopy. The NIRS system can analyze individual seeds or small subsets by detecting the specific absorption signatures of sucrosyl-oligosaccharides, eliminating the need for large representative samples while maintaining measurement precision through direct molecular interaction
Solution Approach 2:
The patent applies localized measurement to specific regions or individual seeds rather than requiring analysis of large bulk samples. By using near-infrared spectroscopy to probe the chemical composition at specific locations, the system obtains representative data from small quantities of material, improving efficiency while maintaining measurement accuracy through targeted analysis
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for accurate measurement of sucrosyl-oligosaccharides with high precision and efficiency, preserving seed viability and enabling rapid analysis of large seed populations, improving the assessment of seed composition and facilitating plant breeding programs.
Implementation Method 1
directing near infrared light from a light source onto a soybean seed to form modified light from the soybean seed
Implementation Method 2
directing near infrared light from a light source onto a soybean seed to form modified light from the soybean seed
Data Source
AI summary
Disclosed are methods and systems for spectral imaging of soybean samples to accurately and non-destructively measure the amount of sucrosyl-oligosaccharide in the soybean samples. Populations containing modified and unmodified soybean seeds and having varying amounts of sucrosyl-oligosaccharides, oil or protein can be sorted and separated and further used in soybean processing or breeding.