Soybean Promoter Sequences for Constitutive Gene Expression
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
There is a limited selection of promoters for genetically modified plants that provide medium to high-level constitutive expression of desirable traits such as insect resistance and herbicide tolerance, particularly in soybean tissues.
Innovation Solution
The use of regulatory elements, including promoters and terminators derived from Glycine species, specifically Glycine max, to construct expression cassettes that drive gene expression in soybean plants, with sequences showing high identity to SEQ ID NOs: 1-57, enabling robust protein production across various tissues.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If existing promoters are used for gene expression in soybean, then expression cassettes can be constructed, but the selection of promoters with medium to high-level constitutive expression is limited
Solution Approach 1:
The patent segments the promoter selection problem by identifying and characterizing multiple distinct promoter sequences (SEQ ID NOs: 1-57) from soybean genomes, dividing the limited option space into numerous selectable alternatives with different expression characteristics
Solution Approach 2:
The patent applies parameter changes by providing promoters with varying expression levels (medium to high), different tissue specificities, and diverse sequence characteristics, allowing selection based on specific application requirements rather than using a single promoter type
2Reliability
If promoters are selected for medium to high-level constitutive expression, then desirable traits such as insect resistance and herbicide tolerance can be achieved, but the choices remain limited
Solution Approach 1:
The patent applies local quality by providing promoters with specific localized characteristics - some promoters are optimized for high-level expression in specific tissues (leaf, root, seed, flower) while others provide constitutive expression across all tissues, allowing matching of promoter properties to specific trait expression needs
Solution Approach 2:
The patent achieves universality by providing a collection of promoters that can all function as constitutive expression drivers for desirable traits, where each promoter serves multiple potential applications (insect resistance, herbicide tolerance, nutritional improvement) depending on the gene of interest
3Productivity
If robust protein production is achieved in all or most soybean tissues, then desirable traits are enhanced, but limited promoter options constrain this achievement
Solution Approach 1:
The patent applies dynamics by providing promoters with different expression dynamics - some promoters drive high-level expression in specific tissues at specific developmental stages, while others provide stable constitutive expression across all tissues and developmental stages, allowing optimization of protein production timing and location
Data Source
AI summary
Provided herein are promoters and related compositions and methods of use. Such promoters are useful for expression cassettes for plants, such as soybean. Such expression cassettes are useful, e.g., to drive expression of trait genes in plants, such as soybean. In some aspects, the disclosure provides an expression cassette comprising a promoter, a 5′ UTR, and an intron.