Soybean Seed Composition via HECT E3 Ligase Modification
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Solution Overview
Problem
Current soybean production does not efficiently produce seeds with high protein content and altered fatty acid profiles, such as increased oleic acid and decreased linolenic and saturated fatty acids, which are desirable for higher-value crops and nutritional benefits.
Innovation Solution
Genomic modification of soybean plants to decrease the expression or activity of HECT E3 ligase polypeptides, resulting in seeds with altered fatty acid compositions and increased protein content, achieved through techniques like gene editing or transformation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional soybean breeding methods are used, then production efficiency is maintained, but seed protein content and altered fatty acid profiles cannot be efficiently achieved
Solution Approach 1:
The patent applies parameter changes by modifying the expression levels of specific genes (FAD2-1A, FAD3-1A, DGAT1b, LEC1, LEC2) to alter seed composition parameters. By changing the expression parameters of these genes through genomic editing, the patent achieves increased protein content and altered fatty acid profiles without traditional time-consuming breeding methods.
2Quantity of substance
If genomic modification is applied to alter fatty acid composition, then oleic acid content increases and linolenic acid decreases, but the complexity of the modification process increases
Solution Approach 1:
The patent applies the extraction principle by specifically targeting and removing or suppressing the function of particular genes (FAD2-1A, FAD3-1A, FAD7, FAD8, DGAT1b, LEC1, LEC2) that control fatty acid metabolism. By extracting or silencing these specific genetic elements, the patent achieves precise control over oleic acid accumulation without needing to modify the entire genome, thereby reducing overall modification complexity.
3Quantity of substance
If multiple genes are modified to achieve high protein and altered fatty acid profiles, then seed composition is improved, but the difficulty of detecting and measuring the modifications increases
Solution Approach 1:
The patent applies feedback principles by establishing specific detection methods and markers to monitor the expression levels of modified genes (FAD2-1A, FAD3-1A, DGAT1b, LEC1, LEC2) and the resulting seed composition changes. This feedback system allows for precise measurement and verification of modification outcomes, facilitating selective breeding and quality control despite multiple gene modifications.
Data Source
AI summary
Soybean seeds with increased protein and having a modified expression or activity of at least one or two HECT E3 ligase polypeptides are provided. Methods for modifying expression or activity of HECT E3 ligase polypeptides and polynucleotides include genome editing to modify the transcription regulatory region or sequence encoding the HECT E3 ligase polypeptides and transformation with recombinant DNA constructs to enhance or suppress expression or activity of the HECT E3 ligase polypeptides. Plants containing the modifications produce seeds with altered composition such as one or more of increased protein, decreased soluble carbohydrate, increased oleic acid, decreased saturated fats such as palmitic and stearic acids, and decreased linoleic or linolenic acid.