Soybean Variety 01073151 Single Locus Conversion

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and herbicide resistance, which are difficult to predict and require extensive research efforts due to the complexity of genetic combinations and environmental factors.

Innovation Solution

The development of the soybean variety 01073151, which incorporates a single locus conversion through genetic transformation, conferring traits like herbicide resistance, disease resistance, and improved nutritional quality, using techniques such as genome editing with engineered nucleases, and the use of specific transgenic genes like MON89788 and MON87708, allowing for precise introduction of desired traits while maintaining the morphological and physiological characteristics of the soybean.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional soybean breeding methods are used to develop new varieties with improved traits, then genetic diversity and trait combinations can be explored, but the process becomes extremely complex, time-consuming, and difficult to predict due to multiple genetic combinations and environmental factors

Engineering Contradiction:
Improvetrait improvementVSAvoidbreeding process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent segments the breeding process by focusing on a single locus conversion rather than attempting to manage multiple genetic combinations simultaneously. This approach divides the complex breeding problem into a manageable single-gene modification task, thereby reducing process complexity while still achieving trait improvement

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent applies preliminary action by using genome editing to pre-introduce the desired trait through single locus conversion before conducting breeding operations. This allows the breeder to start with a plant that already has the desired trait incorporated, eliminating the need to screen through multiple generations and genetic combinations to find the desired trait combination

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If traditional breeding methods are used to combine multiple traits, then genetic stability can be maintained through natural inheritance, but the time required for extensive research and multiple breeding cycles increases significantly

Engineering Contradiction:
Improvegenetic stabilityVSAvoidbreeding cycle time
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The patent performs the genetic modification action in advance through single locus conversion, introducing the desired trait directly into the genome before breeding begins. This preliminary genetic engineering step eliminates the need for multiple breeding cycles to combine traits, significantly reducing the time required while maintaining genetic stability through controlled inheritance

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent substitutes mechanical breeding operations (crossing, selection, and screening across multiple generations) with a direct genetic engineering approach. By using genome editing to introduce traits directly, the patent replaces the time-consuming mechanical breeding process with a more efficient molecular biology-based method

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Adaptability or versatility

If multiple genes are introduced to achieve multiple traits, then trait versatility improves, but the cost and complexity of research and development increases due to the need to manage multiple genetic loci

Engineering Contradiction:
Improvetrait versatilityVSAvoidbreeding operation ease
Core Design Contradiction:
Adaptability or versatilityVSEase of manufacture

Solution Approach 1:

The patent segments the trait introduction process by using single locus conversion for each desired trait. This approach breaks down the complex task of introducing multiple traits into manageable single-gene modifications, making the breeding operations easier to execute while still achieving trait versatility through cumulative single-locus conversions

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent performs preliminary genetic modification through single locus conversion to introduce desired traits before breeding operations begin. This preliminary action eliminates the need for complex multi-gene introgression procedures during breeding, significantly easing the manufacturing and operational complexity while maintaining the ability to achieve multiple traits

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS10952392B2Soybean variety 01073151
Publication Date: 2021.03.23 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01073151. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01073151. Also provided by the invention are tissue cultures of the soybean variety 01073151 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01073151 with itself or another soybean variety and plants produced by such methods.