Soybean XB02F13 Marker-Assisted Trait Stacking

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Solution Overview

Problem

Current soybean breeding processes are time-consuming and resource-intensive, aiming to develop stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved fatty acid profiles, while existing methods face challenges in efficiently combining these traits in a single variety.

Innovation Solution

The development of a novel soybean variety, XB02F13, which is the result of careful breeding and selection, combining traits like resistance to aerial web blight, aphid antibiosis, and improved fatty acid profiles, through a process involving backcrossing and genetic marker-assisted breeding to introduce desired traits and loci.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional soybean breeding methods are used to combine multiple desirable traits, then the variety achieves stability and uniformity in traits, but the breeding process becomes time-consuming and resource-intensive

Engineering Contradiction:
Improvetrait stabilityVSAvoidbreeding duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent employs marker-assisted selection where DNA markers provide feedback on the presence of desired traits during breeding. This allows breeders to track and select for multiple traits simultaneously across generations, significantly reducing the time required to develop stable varieties compared to traditional phenotypic selection methods.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The patent utilizes pre-characterized DNA markers linked to desirable traits before the breeding process begins. These markers serve as preliminary indicators that guide selection decisions from early generations, preventing the need for lengthy field testing and trait verification in later stages.

Inventive Principle:
Principle #10Preliminary action

2Productivity

If multiple desirable traits are combined in a single variety, then the variety achieves superior agronomic performance, but the complexity of the breeding program increases

Engineering Contradiction:
Improvegrain yieldVSAvoidbreeding program complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent divides the complex task of combining multiple traits into manageable segments by using specific DNA markers for each trait. Each marker allows independent tracking and selection of individual traits, breaking down the overall breeding program into discrete, controllable units that can be addressed systematically.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent changes the selection parameter from phenotypic observation to genotypic detection using DNA markers. This parameter change allows simultaneous monitoring of multiple traits through molecular assays, reducing the operational complexity compared to traditional multi-year field trials for each trait.

Inventive Principle:
Principle #35Parameter changes

3Reliability

If extensive field testing is conducted to ensure variety stability, then the variety achieves reliable performance, but the resource consumption increases

Engineering Contradiction:
Improvevariety performanceVSAvoidresource consumption
Core Design Contradiction:
ReliabilityVSQuantity of substance

Solution Approach 1:

The patent replaces mechanical field testing and phenotypic evaluation with molecular biology-based DNA marker analysis. This substitution occurs early in the breeding process, reducing the need for extensive field trials and associated resource consumption while maintaining variety reliability through genetic verification.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS8933304B2Soybean variety XB02F13
Publication Date: 2015.01.13 PIONEER HI BREED INTERNATIONAL INC

AI summary

A novel soybean variety, designated XB02F13 is provided. Also provided are the seeds of soybean variety XB02F13, cells from soybean variety XB02F13, plants of soybean XB02F13, and plant parts of soybean variety XB02F13. Methods provided include producing a soybean plant by crossing soybean variety XB02F13 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety XB02F13, methods for producing other soybean varieties or plant parts derived from soybean variety XB02F13, and methods of characterizing soybean variety XB02F13. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety XB02F13 are further provided.