Soybean XB72D12 Breeding via Marker-Assisted Selection

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Solution Overview

Problem

Current soybean breeding processes are time-consuming and resource-intensive, aiming to develop stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved fatty acid profiles, while existing methods struggle to efficiently combine these traits in a single variety.

Innovation Solution

The development of the soybean variety XB72D12, which is the result of careful breeding and selection, combining traits like resistance to aerial web blight, aphid antibiosis, and improved fatty acid profiles, through a process involving backcrossing and genetic marker-assisted breeding to introduce desired traits and achieve phenotypic stability.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional soybean breeding methods are used to combine multiple desirable traits, then trait combination is achieved, but the breeding process becomes time-consuming and resource-intensive

Engineering Contradiction:
Improvetrait combinationVSAvoidbreeding process duration
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent employs marker-assisted selection where DNA markers provide feedback on the presence of desired traits during breeding. This allows breeders to track and select for multiple traits simultaneously without waiting for phenotypic expression, significantly reducing breeding time while maintaining effective trait combination.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The patent replaces traditional mechanical phenotypic selection with molecular marker-based genotypic selection. Instead of observing physical traits that require growing plants to maturity, the method uses DNA markers to detect trait presence at the molecular level, accelerating the breeding process while achieving the same trait combination goals.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Reliability

If multiple traits are combined in a single soybean variety, then variety improvement is achieved, but the complexity of the breeding program increases

Engineering Contradiction:
Improvevariety improvementVSAvoidbreeding program complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent segments the breeding program into distinct phases: initial parental selection based on marker profiles, intermediate backcrossing generations with selective marker screening, and final variety development. This segmentation manages complexity by breaking down the multi-trait combination process into manageable steps with clear decision points at each stage.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent uses DNA markers as intermediaries between the breeder and the traits of interest. These markers serve as proxies that simplify the selection process, allowing breeders to indirectly select for complex trait combinations without directly observing or measuring each individual trait, thereby reducing program complexity.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Stability of the object's composition

If phenotypic stability is achieved through careful selection, then trait uniformity is improved, but the breeding process requires multiple generations and extended time

Engineering Contradiction:
Improvetrait uniformityVSAvoidbreeding efficiency
Core Design Contradiction:
Stability of the object's compositionVSProductivity

Solution Approach 1:

The patent performs preliminary selection using DNA markers at early breeding stages, identifying and selecting plants with the desired genetic composition before phenotypic expression occurs. This preliminary genotypic selection ensures that only plants with the correct trait combination advance to subsequent generations, achieving phenotypic stability more efficiently by preventing unwanted variation from propagating.

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS9179619B1Soybean variety XB72D12
Publication Date: 2015.11.10 PIONEER HI BREED INTERNATIONAL INC

AI summary

A novel soybean variety, designated XB72D12 is provided. Also provided are the seeds of soybean variety XB72D12, cells from soybean variety XB72D12, plants of soybean XB72D12, and plant parts of soybean variety XB72D12. Methods provided include producing a soybean plant by crossing soybean variety XB72D12 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety XB72D12, methods for producing other soybean varieties or plant parts derived from soybean variety XB72D12, and methods of characterizing soybean variety XB72D12. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety XB72D12 are further provided.