Soybean XB85F13 Marker-Assisted Breeding for Disease Resistance

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Solution Overview

Problem

Current soybean breeding processes are time-consuming and resource-intensive, aiming to develop stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved fatty acid profiles, while existing methods face challenges in efficiently combining these traits in a single variety.

Innovation Solution

The development of the soybean variety XB85F13, which is the result of careful breeding and selection, combining traits like resistance to aerial web blight, aphid antibiosis, and improved fatty acid profiles, through a process involving backcrossing and genetic marker-assisted breeding to introduce desired traits and achieve phenotypic stability.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional soybean breeding methods are used to combine multiple desirable traits, then the variety achieves disease resistance and improved yield, but the breeding process becomes extremely time-consuming and resource-intensive

Engineering Contradiction:
Improvedisease resistanceVSAvoidbreeding process duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent employs preliminary action by using molecular markers to identify and select plants with desired traits at early stages of breeding, rather than waiting for phenotypic expression. This allows breeders to make informed selection decisions before the actual trait expression occurs, significantly reducing the time required to develop varieties with multiple desirable traits including disease resistance

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent introduces molecular markers as an intermediary tool that mediates between the genotype and phenotype. These markers serve as indicators that allow breeders to track and select for multiple traits simultaneously without having to wait for each trait to manifest phenotypically, thereby accelerating the breeding process while maintaining reliability of trait inheritance

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If multiple desirable traits are combined into a single soybean variety, then the variety achieves superior agronomic performance and yield, but the breeding complexity and resource requirements increase significantly

Engineering Contradiction:
Improvegrain yieldVSAvoidbreeding program complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent applies segmentation by breaking down the complex task of combining multiple traits into manageable segments using molecular markers. Each marker corresponds to specific genetic loci associated with particular traits, allowing breeders to address and select for individual traits or groups of traits separately through marker-assisted selection, rather than attempting to select for all traits simultaneously through traditional phenotypic evaluation

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent replaces the mechanical system of traditional phenotypic selection with a molecular-level selection system. Instead of visually evaluating and selecting plants based on physical characteristics, the patent uses molecular markers to detect and select for desired genetic traits at the DNA level, thereby reducing the complexity of the breeding program while achieving the same or better results

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Stability of the object's composition

If extensive breeding and selection processes are used to achieve phenotypic stability, then the variety achieves uniformity and stability in its traits, but the development time extends to six to twelve years

Engineering Contradiction:
Improvetrait stabilityVSAvoidvariety development time
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The patent uses preliminary action by performing molecular marker analysis at early generations to predict and ensure phenotypic stability before advancing to later generations. This allows breeders to identify and eliminate unstable lines early in the breeding process, preventing the need for extensive multi-year testing while still achieving the desired trait stability in the final variety

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent implements feedback mechanisms through molecular marker analysis that provide real-time information about the genetic composition and stability of breeding lines. This feedback allows breeders to make informed decisions about which lines to advance or eliminate at each generation, accelerating the achievement of phenotypic stability while reducing the overall development time from six to twelve years

Inventive Principle:
Principle #23Feedback

Data Source

PatentUS8822774B1Soybean variety XB85F13
Publication Date: 2014.09.02 PIONEER HI BREED INTERNATIONAL INC

AI summary

A novel soybean variety, designated XB85F13 is provided. Also provided are the seeds of soybean variety XB85F13, cells from soybean variety XB85F13, plants of soybean XB85F13, and plant parts of soybean variety XB85F13. Methods provided include producing a soybean plant by crossing soybean variety XB85F13 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety XB85F13, methods for producing other soybean varieties or plant parts derived from soybean variety XB85F13, and methods of characterizing soybean variety XB85F13. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety XB85F13 are further provided.