Soybean Yellow Flash Phenotype Marker Selection

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Solution Overview

Problem

Soybean varieties with a transgene conferring glyphosate tolerance can exhibit an undesirable 'Yellow Flash' phenotype, leading to visually displeasing off-type yellow leaf color, with varying degrees of susceptibility to high glyphosate doses, making it challenging to breed for consistent tolerance and resistance.

Innovation Solution

Identification and introgression of a genomic region associated with a 'no flash' phenotype, using specific markers to select for favorable alleles and eliminate unfavorable ones, allowing for the development of soybean lines that remain resistant to high glyphosate doses without the yellow flash phenotype.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If soybean varieties are bred for glyphosate tolerance using transgenes, then glyphosate tolerance is improved, but yellow flash phenotype occurs

Engineering Contradiction:

Improveglyphosate toleranceVSAvoidyellow flash phenotype

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent extracts and identifies the specific chromosomal region (linkage group L, approximately 10-20 cM from the centromere) that contains the yellow flash phenotype, allowing breeders to separate this harmful trait from the desirable glyphosate tolerance transgene through targeted selection and introgression of the no-flash genomic region

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent applies local quality by identifying a specific localized genomic region (linkage group L) that controls the yellow flash phenotype, enabling precise breeding interventions in only that specific area while maintaining the overall glyphosate tolerance trait across the rest of the genome

Inventive Principle:

Principle #3Local quality

2Productivity

If marker-assisted selection is used to select for no flash phenotype, then breeding efficiency is improved, but marker identification and validation complexity increases

Engineering Contradiction:

Improvebreeding efficiencyVSAvoidmarker identification and validation complexity

Core Design Contradiction:

ProductivityVSDevice complexity

Solution Approach 1:

The patent performs preliminary action by pre-identifying and validating the molecular markers (such as SSR markers) associated with the no-flash phenotype before they are needed in the breeding program, establishing a foundation of verified markers that can be directly applied in marker-assisted selection without requiring complex real-time validation

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The patent uses molecular markers as intermediary tools to indirectly select for the no-flash phenotype without needing to directly observe or measure the phenotype itself, simplifying the breeding process by using easy-to-detect DNA markers as proxies for the complex phenotypic trait

Inventive Principle:

Principle #24Intermediary (Mediator)

Data Source

PatentUS9617605B2Molecular markers associated with yellow flash in glyphosate tolerant soybeans

Publication Date: 2017.04.11 MONSANTO TECHNOLOGY LLC

AI summary

The present invention provides methods and compositions for the identification and selection of loci modulating phenotypic expression of a herbicide tolerance trait in plant breeding. In addition, methods are provided for screening germplasm entries for the performance and expression of this trait.