SP3D Promoter Sequence Reduces Sympodial Index in Tomato
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Solution Overview
Problem
Current tomato breeding methods have limitations in increasing crop yield, with gains becoming smaller due to a lack of understanding about the genetic factors determining tomato yield, particularly in achieving a sympodial index of 2, which is associated with higher yields.
Innovation Solution
Introduction of a SP3D promotor sequence with a specific CA motif upstream of the SP3D gene, derived from Solanaceae family members like Solanum pennellii, to reduce the sympodial index from 3 to 2, thereby increasing crop yield by altering the plant architecture and enhancing fruit production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If current tomato breeding methods are used, then crop yield can be increased, but the gains become smaller due to lack of understanding about genetic factors
Solution Approach 1:
The patent uses the SP3D promotor sequence as an intermediary genetic element to control the expression of the SP3D gene. This promotor sequence acts as a mediator that regulates plant architecture and sympodial index, thereby increasing crop yield without requiring comprehensive understanding of all genetic factors involved in tomato production.
2Productivity
If SP3D promotor sequence is introduced to reduce sympodial index from 3 to 2, then crop yield increases, but plant architecture is altered
Solution Approach 1:
The patent changes the sympodial index parameter from 3 to 2 by introducing the SP3D promotor sequence. This parameter change in plant architecture directly leads to increased crop yield by optimizing the distribution of fruits per truss and improving overall plant productivity.
Data Source
AI summary
Presented herein is a SP3D promotor sequence, capable of directing transcription of a downstream SP3D gene that is operably linked to said promotor sequence, wherein the promotor sequence is derived from a species of the Solanaceae family having a sympodial index of 2, for reducing sympodial index in plants having a sympodial index of 3 or more. Also presented herein are methods for providing plants of the Solanaceae family, in particular S. lycopersicum, having a reduced sympodial index including the steps of introducing into the genome of said plants a promotor sequence in operable linkage with a downstream SP3D gene, or introducing into said plants a gene construct, such that the sympodial index of the plant with the promotor sequence or gene construct is reduced as compared to the plant without said promotor sequence or gene construct, and to plants obtainable by said methods.