Spatial Multi-Omics Probes for In Situ IBD Biomarker Detection
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Solution Overview
Problem
Current spatial multi-omic technologies for inflammatory bowel disease (IBD) are costly, low-throughput, and inflexible, making them impractical for large-scale clinical studies, and often require multiple platforms to analyze both proteins and transcripts, increasing costs and sample requirements.
Innovation Solution
Development of spatial multi-omics methods and systems for detecting IBD-associated biomolecules, including RNA transcripts and proteins, using padlock probes and G4X spatial multi-omics data, enabling efficient analysis on solid supports or tissue sections in situ.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If existing spatial multi-omic platforms are used to analyze IBD biomolecules, then measurement precision is maintained, but cost increases and throughput decreases
Solution Approach 1:
The patent combines protein and transcript detection into a single spatial multi-omic platform, eliminating the need for separate platforms and thereby increasing throughput while maintaining detection precision through integrated assay design
Solution Approach 2:
The invention creates a universal platform capable of detecting multiple analyte types (proteins and transcripts) simultaneously with the same core technology, enabling high-throughput analysis without sacrificing measurement accuracy
2Measurement precision
If multiple platforms are used to track different analyte types, then measurement precision is improved, but device complexity and cost increase
Solution Approach 1:
The patent develops a single universal platform that can detect both proteins and transcripts using the same core technology, eliminating the need for multiple specialized platforms and thereby reducing device complexity while maintaining precision
Solution Approach 2:
The invention segments the detection process into modular components (probe design, signal amplification, detection) that can handle different analyte types within a unified framework, simplifying overall system complexity
3Measurement precision
If extensive endoscopic sampling is performed, then measurement precision is improved, but loss of time and cost increase
Solution Approach 1:
The patent combines multiple detection capabilities into a single assay performed on limited tissue samples, eliminating the need for extensive sampling while maintaining comprehensive disease characterization through multiplexed detection
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enhances the speed and efficiency of target detection in IBD analysis, reducing costs and improving the scalability of clinical studies by providing comprehensive insights into cellular processes and biomarker discovery.
Implementation Method 1
padlock probes
Implementation Method 2
G4X spatial multi-omics data
Data Source
AI summary
Disclosed herein, inter alia, are oligonucleotide probes, methods, and kits useful for amplifying and detecting target nucleic acids in situ.