Spatial Sequencing Using Location-Specific and Multiple-Location Markers
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Solution Overview
Problem
Existing spatial-sequencing methods are limited in their ability to efficiently associate analytes from a sample with location-specific markers and multiple-location markers, which hinders precise spatial analysis.
Innovation Solution
A spatial-sequencing method that involves causing analytes from a sample to bind to location-specific markers and multiple-location markers, where each analyte type corresponds to a specific analyte type, and each marker type is associated with distinct locations and marker types, respectively.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If existing spatial-sequencing methods are used to associate analytes with location-specific markers, then the basic spatial sequencing function is achieved, but the efficiency and precision of spatial analysis are limited
Solution Approach 1:
The patent divides the spatial sequencing process into distinct functional segments: location-specific markers (LSMs) for initial spatial tagging, multiple-location markers (MLMs) for cross-location association, analyte binding, and computational spatial reconstruction. This segmentation allows each component to be optimized independently, improving both efficiency through parallel processing and precision through multi-stage verification.
Solution Approach 2:
The patent introduces multiple-location markers as intermediary elements that bridge multiple locations simultaneously. These MLMs act as mediators between different spatial locations and analytes, enabling efficient cross-location associations and improving the precision of spatial analysis by providing multiple reference points for accurate position determination.
2Measurement precision
If multiple-location markers are introduced to improve spatial analysis precision, then measurement precision improves, but device complexity increases
Solution Approach 1:
The multiple-location markers are designed with universal functionality to associate with multiple different location-specific markers across different locations. Each MLM serves multiple purposes: it acts as a spatial reference, enables cross-location associations, and provides multi-point verification for position determination. This multi-functionality reduces the need for separate specialized components, thereby managing system complexity while improving precision.
Solution Approach 2:
The patent employs parameter changes in the marker system design, where markers are characterized by specific parameters such as location coordinates, marker types, and association strengths. By systematically varying these parameters across different marker instances, the system achieves high measurement precision through computational analysis of parameter patterns, avoiding the need for overly complex physical structures.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables precise spatial analysis by effectively associating analytes with location-specific and multiple-location markers, allowing for the determination of analyte positions and abundances at specific locations within a sample.
Implementation Method 1
causing the plurality of analytes to bind to the respective location-specific markers
Data Source
AI summary
Spatial-sequencing methods are disclosed.