Microparticle Spectrometer Photoelectric Array Channel Calibration
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Solution Overview
Problem
In spectrum type microparticle measurement devices, the signal-to-noise ratio (SNR) of channels with small outputs deteriorates, and the quantitativeness of measured data is lost due to unsaturated electric circuits and neglect of wavelength characteristics of light sources, spectrometers, and PMT photoelectric conversion films, leading to inconsistent reference spectra.
Innovation Solution
A microparticle measurement spectrometer with a photoelectric conversion array having multiple light receiving elements with different detection wavelength ranges, which adjusts independent gains and output values based on optical information from fluorescent reference particles and microparticles to ensure uniform output across channels when light intensity is consistent per unit wavelength, thereby calibrating the system to achieve consistent measurement results.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If multiple fluorescent dyes are used for multicolor analysis, then analysis capability is improved, but light leakage from non-target dyes into photoelectric converters increases, causing a decrease in analysis accuracy
Solution Approach 1:
The photoelectric converter is divided into multiple channels, each responsible for detecting a specific wavelength range. This segmentation allows simultaneous detection of multiple fluorescent dyes while preventing cross-contamination between channels, as each channel is optimized for its designated wavelength range.
Solution Approach 2:
Each channel in the photoelectric converter array is assigned specific wavelength characteristics and detection ranges tailored to particular fluorescent dyes. This local optimization ensures that each channel has high sensitivity for its target dye while inherently rejecting light from other wavelength ranges, eliminating the need for complex fluorescence correction.
2Device complexity
If conventional photoelectric converters are used with multiple channels, then device complexity is reduced, but uniformity of output across channels deteriorates, causing inconsistency in measurement results
Solution Approach 1:
The invention changes the fundamental parameters of the photoelectric converter by using an array of multiple converters with different wavelength sensitivities rather than a single converter. Each element in the array has optimized spectral response characteristics, and through calibration procedures, uniform output levels are achieved across all channels while maintaining wavelength-specific detection capabilities.
3Measurement precision
If gain is increased to improve signal detection, then sensitivity is improved, but signal-to-noise ratio in channels with small outputs deteriorates
Solution Approach 1:
Each channel in the photoelectric converter array is optimized with appropriate gain settings and wavelength filtering specific to its detection range. This local optimization ensures that each channel operates at optimal sensitivity for its target wavelength while maintaining adequate signal-to-noise ratio, avoiding the need to uniformly increase gain across all channels which would amplify noise.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach ensures uniform output across all channels, preventing SNR deterioration and maintaining data quantitativeness by calibrating the system to achieve consistent measurement results comparable to a reference device, even under varying conditions.
Implementation Method 1
a spectroscopic element that disperses light emitted from microparticles flowing through a flow path
Implementation Method 2
a photoelectric conversion array that has a plurality of light receiving elements having different detection wavelength ranges and converts optical information obtained by the light receiving elements into electrical information
Implementation Method 3
the microparticles are irradiated with laser light or the like, and fluorescence or scattered light emitted from each microparticle is detected
Data Source
AI summary
A microparticle measurement spectrometer includes a spectroscopic element that disperses light emitted from microparticles flowing through a flow path, and a photoelectric conversion array that has a plurality of light receiving elements having different detection wavelength ranges and converts optical information obtained by the light receiving elements into electrical information, in which the photoelectric conversion array has a uniform output of all channels when light with which the amount of light per unit wavelength becomes same is incident.