Spectrometric Measurement Device Optimizing Wavelength Selection
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Solution Overview
Problem
Conventional spectrometric measurement devices used in liquid chromatography do not consider the change in baseline noise during the mobile phase supply, leading to determined wavelengths that may not maximize the signal-to-noise (S/N) ratio, thus not minimizing the detection limit.
Innovation Solution
A spectrometric measurement device with a data memory and sensitivity index estimator that calculates the degree of interaction between the objective component and irradiation light, and estimates noise due to the solvent, to determine the optimal wavelength for maximizing the S/N ratio and minimizing the detection limit.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional wavelength determination methods are used (based on maximum fluorescence intensity or absorbance), then the measurement process is simple, but the S/N ratio is not maximized and detection limit is not minimized
Solution Approach 1:
The patent applies preliminary action by performing baseline noise measurements during the mobile phase supply period before actual sample analysis. The system pre-acquires solvent baseline data and pre-calculates optimal wavelengths, so that when real measurement begins, the system already has noise characteristics and can immediately determine optimal wavelengths for maximizing S/N ratio without adding complexity to the main measurement process
Solution Approach 2:
The system implements feedback by continuously monitoring baseline noise levels during mobile phase supply and using this information to dynamically adjust wavelength selection. The sensitivity index calculation incorporates real-time noise data from the solvent baseline, creating a feedback loop that optimizes measurement parameters based on actual system conditions rather than relying on fixed predetermined wavelengths
2Measurement precision
If optimal wavelengths are determined without considering baseline noise, then the measurement setup is straightforward, but the detection limit cannot be minimized
Solution Approach 1:
The patent extracts baseline noise information from the solvent baseline measurements taken during mobile phase supply. By separating the noise characterization step from the main measurement process and extracting quantitative noise data, the system can use this extracted information to optimize wavelength selection and minimize detection limits without interfering with the primary analytical function
Solution Approach 2:
The system applies parameter changes by using the extracted baseline noise information to dynamically adjust the wavelength parameter. Instead of using fixed wavelengths based on maximum intensity alone, the system modifies wavelength selection based on noise characteristics, transforming the wavelength from a static parameter to a dynamically optimized one that accounts for baseline variations
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables the determination of the wavelength at which the S/N ratio is maximized and the detection limit is minimized, optimizing the measurement conditions for detecting the objective component.
Implementation Method 1
an excitation spectroscopic system for separating a predetermined wavelength of light from the light generated by a light source
Implementation Method 2
a fluorescent spectroscopic system for separating a predetermined wavelength of light from the light emitted from the sample upon irradiation with the excitation light
Implementation Method 3
a photodetector for detecting the light separated by the fluorescent spectroscopic system and producing a signal corresponding to the amount of the detected light
Implementation Method 4
an absorbance determination device normally has an irradiation optical system for separating a predetermined wavelength of light from the light generated by a light source and irradiating a sample with the separated light as the irradiation light and a photodetector for detecting the light that has passed through the sample
Data Source
AI summary
A spectrometric measurement device capable of determining an optimal wavelength for detecting an objective component is provided. One mode of the present invention is a fluorescence measurement device for casting an excitation light of a predetermined wavelength into or onto a sample and detecting a predetermined wavelength of light contained in the fluorescence generated from the sample irradiated with the excitation light.