High-Throughput 3D Cell Spheroid Culture Chip
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Solution Overview
Problem
Current three-dimensional cell culture methods, such as cell suspension culture bottles, hanging drop methods, and ultra-low adhesion culture dishes, face challenges like low spheroidization efficiency and inconvenient operation, limiting their effectiveness in forming high-throughput 3D cell spheroids.
Innovation Solution
A high-throughput 3D cell spheroid culture chip is developed, comprising a polycarbonate substrate with pressed probe arrays and a method for preparing it, which involves pressing probe arrays into the substrate, coating with a solution, and inoculating cells to form spheroids.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional three-dimensional cell culture methods (cell suspension culture bottles, hanging drop methods, ultra-low adhesion culture dishes) are used, then cell culture can be performed, but spheroidization efficiency is low and operation is inconvenient
Solution Approach 1:
The invention uses probe arrays as molds to copy the desired spheroid shape directly into the culture substrate. The probe arrays are pressed into the polycarbonate substrate to create imprinted cavities that serve as templates for spheroid formation, enabling high-throughput production of uniform cell spheroids while simplifying the操作流程
Solution Approach 2:
The culture system is segmented into multiple independent probe arrays, each capable of forming spheroids simultaneously. The probe arrays are arranged in an organized pattern on the substrate, allowing parallel processing of multiple cell spheroids in a single experiment, thereby significantly improving productivity
2Productivity
If probe arrays are pressed into polycarbonate substrate to form culture chip, then high-throughput spheroid formation is achieved, but device fabrication complexity increases
Solution Approach 1:
The probe arrays are prepared and positioned in advance before being pressed into the polycarbonate substrate. The imprinted patterns are created beforehand using simple pressing operations, and the substrate is pre-drilled with through-holes to facilitate probe insertion and solution penetration, simplifying the overall fabrication process
Solution Approach 2:
The invention optimizes pressing parameters such as pressure, temperature, and time to achieve effective imprinting without requiring complex fabrication equipment. The probe array geometry and material properties are adjusted to enable simple pressing operations that create functional culture structures
Data Source
AI summary
The present disclosure provides a high-throughput 3D cell spheroid culture chip, the preparation process and uses thereof. Through various efficacy experiments in the present disclosure, first evidence of using hydrogels derived from decellularized liver tissue as a self-healing biomaterial to reduce damage to damaged hepatocytes and enhance liver function in vitro is provided. Integrating endothelial cell-covered hepatocyte spheroids into DLM-CP hydrogels is a promising approach to develop microbial liver tissue, providing a potential solution for liver fibrosis recovery and promoting cell-level therapy. DLM-CP hydrogels show great potential for cell encapsulation for therapeutic purposes in future clinical settings and may be applied to ultra-high-throughput three-dimensional cell spheroid culture chips. It is used to create artificial tissues and organs, becoming a high-value tool widely used in biomedical research and pharmaceutical fields.