Spiked Matrix Standards for Quantitative Analysis
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Solution Overview
Problem
Current methods for quantitative analysis of analytes in solid samples or formulations are time-consuming, prone to human error, and lack a ready-to-use format that incorporates both spiked matrix components and internal standards, leading to inaccuracies and inefficiencies in industries such as forensic, clinical, and environmental testing.
Innovation Solution
A high and medium throughput multicomponent quantitative assay kit in a ready-to-extract format that includes spiked matrix components and extraction solutions, with internal standards, allowing for precise and accurate analysis of analytes like phytocannabinoids, terpenes, pesticides, and heavy metals by providing spiked matrix calibration and quality control standards, blank matrix standards, and extraction solutions for sample preparation and analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional quantitative assay methods are used with separate preparation of standard solutions and blank matrices, then measurement precision can be maintained, but the device complexity and time consumption increase significantly
Solution Approach 1:
The invention combines the blank matrix material and spiked analyte standards into a single integrated kit format. The kit includes both the blank matrix (free of analytes) and the spiked matrix (containing known quantities of analytes) as separate but coordinated components, eliminating the need for separate preparation of standard solutions and blank matrices. This merging reduces device complexity while maintaining measurement precision through consistent matrix composition.
Solution Approach 2:
The invention performs preliminary preparation by pre-formulating the blank matrix and spiked matrix components in the kit before use. The matrix material is prepared in advance with precise composition, and the analyte spiking is pre-calculated and packaged. This preliminary action eliminates time-consuming preparation steps during actual analysis while maintaining the precision required for quantitative assays.
2Measurement precision
If multiple liquid transfers are performed for simultaneous quantitation of multiple analytes, then measurement completeness improves, but the risk of human error increases
Solution Approach 1:
The invention enables the assay system to serve itself by providing pre-prepared matrix components that require minimal handling. The kit includes pre-formulated blank and spiked matrices that can be directly used without extensive liquid transfer operations. This self-service approach maintains quantification completeness for multiple analytes while significantly reducing opportunities for human error in liquid handling.
Solution Approach 2:
The invention performs preliminary preparation of all necessary matrix components and analyte combinations in the kit formulation stage. Multiple analyte standards are pre-combined with the matrix material in precise ratios before packaging. This preliminary action ensures complete quantitation of multiple analytes while eliminating the need for numerous liquid transfer steps that would increase error risk.
3Loss of time
If blank matrix is used without subjecting to all sample processing steps, then time consumption is reduced, but measurement precision deteriorates due to unaccounted processing bias
Solution Approach 1:
The invention changes the parameter of matrix preparation by providing pre-processed matrix components that have already undergone the necessary processing steps. The blank and spiked matrices are prepared in advance with consistent processing parameters, ensuring that any processing bias is accounted for in both the blank and sample measurements. This allows rapid analysis while maintaining precision through consistent processing conditions.
Data Source
AI summary
The present disclosure provides a set of spiked matrix standards comprising: one or more carriers, each carrier comprising: (a) a reference standard analyte in an amount that is different in each carrier; and (b) a blank matrix that is in admixture or in contact with the reference standard analyte. Methods for preparing and quantitating an analyte in a test sample comprises (a) providing one or more test samples to be tested for the presence of the analyte (b) providing a set of spiked matrix standards; (c) processing the standards and test samples and (d) quantitating the amount of analyte in each of the standards and test samples.