Spinach p10 Locus Non-R-Gene Resistance to Downy Mildew

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Solution Overview

Problem

Spinach plants face significant challenges due to the rapid evolution of downy mildew pathogen races, rendering R-gene mediated resistance durable for only a short period, necessitating a more stable resistance mechanism.

Innovation Solution

Development of spinach plants with a non-R-gene mediated resistance conferred by the p10 locus, which provides broad-spectrum resistance to multiple downy mildew races by introgressing a specific chromosomal interval, and can be combined with dominant R-genes to enhance durability.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If R-gene mediated resistance is used to protect spinach plants against downy mildew, then resistance is achieved, but the durability is short due to rapid pathogen evolution

Engineering Contradiction:
Improveresistance durabilityVSAvoidpathogen race evolution
Core Design Contradiction:
ReliabilityVSAdaptability or versatility

Solution Approach 1:

The patent segments the resistance mechanism into two independent components: the p10 locus providing stable baseline resistance and R-genes providing enhanced protection. This segmentation allows each component to function independently, preventing the pathogen from overcoming both simultaneously and thereby extending durability

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent creates a composite resistance system by combining the p10 locus with dominant R-genes in the same plant. This composite approach integrates two different resistance mechanisms (non-R-gene mediated and R-gene mediated) to produce synergistic effects that slow pathogen evolution and extend resistance durability

Inventive Principle:
Principle #40Composite materials

2Adaptability or versatility

If non-R-gene mediated resistance via p10 locus is used, then broad-spectrum resistance to multiple races is achieved, but resistance level is intermediate rather than complete

Engineering Contradiction:
Improvebroad-spectrum resistanceVSAvoidresistance level
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The patent merges the broad-spectrum p10 locus with race-specific R-genes to create a unified resistance system. The p10 locus provides coverage against multiple races while R-genes provide complete protection against specific races, and their combination achieves both broad spectrum and high resistance level simultaneously

Inventive Principle:
Principle #5Merging (Combining)

Data Source

PatentUS11751523B2Non R-gene mediated resistance
Publication Date: 2023.09.12 RIJK ZWAAN ZAADTEELT & ZAADHANDEL BV

AI summary

The invention relates to plants and plant parts, in particular spinach plants (Spinacia oleracea L.), which are resistant to Peronospora farinosa f. sp. spinaciae. The invention also relates to seeds capable of producing Peronospora farinosa f. sp. spinaciae resistant plants. The invention further relates to methods for obtaining said plants with altered genotypes and seeds thereof, which are resistant to Peronospora farinosa f sp. spinaciae.