Spinach Line SMB66-1114F Downy Mildew Resistance Breeding

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Solution Overview

Problem

Current spinach breeding programs lack new lines with improved traits such as increased yield, resistance to diseases, and adaptability to various environmental conditions, which limits crop quality and yield for farmers and consumers.

Innovation Solution

Development of the spinach line SMB66-1114F, which is homozygous and exhibits desirable traits like mid-late bolting, spineless seeds, fast growth, and resistance to downy mildew, achieved through pedigree selection and potentially enhanced by genetic transformation techniques to introduce traits like herbicide tolerance and disease resistance.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional breeding methods are used to develop spinach lines, then uniform varieties can be produced through self-pollination and selection, but the process is time-consuming and lacks new lines with improved traits

Engineering Contradiction:
Improveuniformity of varietyVSAvoidbreeding program duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent develops inbred lines through multiple generations of self-pollination and selection before creating hybrids, preparing uniform parental lines in advance. This preliminary development of homozygous inbred lines (requiring 6-8 generations) ensures uniformity in the final hybrid variety while organizing the breeding process into manageable stages that can be parallelized

Inventive Principle:
Principle #10Preliminary action

2Adaptability or versatility

If existing spinach lines are used, then current varieties are available for planting, but they lack resistance to diseases like downy mildew and have limited adaptability to environmental conditions

Engineering Contradiction:
Improvedisease resistance and environmental adaptabilityVSAvoidcrop yield and quality
Core Design Contradiction:
Adaptability or versatilityVSQuantity of substance

Solution Approach 1:

The patent combines multiple desirable traits from different parental lines into a single hybrid variety. Specifically, it merges disease resistance (downy mildew resistance from one parent), adaptability to environmental conditions, and high yield potential from another parent, creating a unified hybrid line that expresses all these improved traits simultaneously

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The hybrid spinach line represents a composite genetic structure combining DNA from two different inbred lines. This genetic composite allows the plant to express a broader range of beneficial traits including multiple disease resistances and environmental adaptabilities that would not be present in either parental line alone

Inventive Principle:
Principle #40Composite materials

3Productivity

If cross-pollination between different varieties is performed, then hybrid vigor and improved traits can be achieved, but the resulting population is genetically non-uniform and performance is unpredictable

Engineering Contradiction:
Improvehybrid vigor and improved yieldVSAvoidgenetic uniformity
Core Design Contradiction:
ProductivityVSManufacturing precision

Solution Approach 1:

The patent performs preliminary self-pollination and selection for 6-8 generations to create homozygous inbred lines before crossing. This preliminary purification ensures that each parental line is genetically uniform, so when crossed, they produce F1 hybrids that are uniformly heterozygous and predictably uniform in performance

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent changes the genetic parameter from homozygous (inbred lines) to heterozygous (F1 hybrids) through controlled crossing. This parameter change at the genetic level produces hybrid vigor while maintaining uniformity because all F1 individuals receive the same combination of alleles from their standardized parental lines

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS7939720B2Spinach line SMB66-1114F
Publication Date: 2011.05.10 SEMINIS VEGETABLE SEEDS INC

AI summary

The invention provides seed and plants of the spinach line designated SMB66-1114F. The invention thus relates to the plants, seeds and tissue cultures of spinach line SMB66-1114F, and to methods for producing a spinach plant produced by crossing a plant of spinach line SMB66-1114F with itself or with another spinach plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of spinach line SMB66-1114F, including the fruit and gametes of such plants.