SPR Biosensor Calibration Method for Accurate Analyte Concentration
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Solution Overview
Problem
Current surface plasmon resonance (SPR) biosensor systems face challenges in achieving accurate measurements due to instrument-dependent constants that vary with each specific instrument, flow system, and sensor surface, leading to inconsistencies in calculating analyte concentration.
Innovation Solution
A method is developed to adjust instrument-dependent parameters for each specific SPR biosensor system, flow system, and sensor surface, allowing for more accurate calculations by determining and using a new constant that converts response units into mass units, thereby improving measurement accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a universal constant is used for converting response units to mass units in SPR biosensor systems, then the device complexity is reduced and ease of operation is improved, but measurement precision deteriorates due to instrument-dependent variations
Solution Approach 1:
The patent applies parameter changes by adjusting the conversion constant based on instrument-specific parameters. Instead of using a fixed universal constant, the system determines instrument-dependent parameters (such as flow cell characteristics, sensor surface properties, and optical alignment factors) and uses these to calculate a customized conversion constant for each instrument configuration, thereby improving measurement precision while accounting for system variations
Solution Approach 2:
The patent implements feedback by using measured data from the instrument to refine and adjust the conversion constant. The system measures instrument-dependent parameters, calculates the appropriate conversion constant, and applies it to subsequent measurements. This feedback loop ensures that the conversion constant remains accurate for each specific instrument configuration without requiring complex manual calibration procedures
2Measurement precision
If instrument-dependent parameters are adjusted for each specific instrument, then measurement precision is improved, but device complexity and ease of operation worsen
Solution Approach 1:
The patent applies self-service by enabling the instrument to automatically determine its own instrument-dependent parameters and calculate the appropriate conversion constant without requiring manual intervention or complex user configuration. The system performs self-calibration by measuring its own characteristics and adjusting the conversion parameters accordingly, thereby maintaining measurement precision while preserving ease of operation
Solution Approach 2:
The patent implements preliminary action by determining the instrument-dependent parameters and calculating the conversion constant in advance, before actual sample measurements are performed. This preliminary calibration step is integrated into the system initialization process, so that when users perform measurements, the correct conversion constant is already in place, eliminating the need for users to manually adjust parameters during operation
Data Source
Figure 1
AI summary
A method for determining instrument depending parameters of a surface plasmon resonance (SPR) biosensor system comprising the steps of:a) identifying (S1) an analyte for use in a calibration step, said analyte having known molecular weight, known diffusion constant, known refractive index increment and a known concentration; b) measuring active concentration (S3) of the analyte by the use of the SPR biosensor system according to a known method, preferably according to calibration-free concentration analysis, using the analyte identified in step a) and a chip to which the analyte can bind, said known method including the use of an instrument depending constant that is not adopted for each specific instrument, chip and flow cell combination; c) comparing (S5) the known concentration of the analyte with the active concentration retrieved in step b); and d) adjusting (S7) the instrument depending constant used in the known method for measuring active concentration according to a result of the comparing in step c).