Surface Plasmon Resonance Detection Device Temperature Control
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Solution Overview
Problem
Existing surface plasmon resonance detection devices face challenges in maintaining consistent temperature conditions, affecting the sensitivity and accuracy of detecting minute amounts of substances like proteins and DNA due to temperature-dependent primary and secondary reactions and fluorescence intensity.
Innovation Solution
A detection device and method that includes a detection chip with a prism, metal film, and a base body forming a liquid reservoir, along with a heating section to maintain constant temperature, ensuring precise control over the reaction site, and a configuration that avoids interfering with the light path to prevent temperature fluctuations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If temperature control is not implemented, then the device structure remains simple, but the detection sensitivity and quantitative accuracy deteriorate due to temperature-dependent reaction rates and fluorescence intensity variations
Solution Approach 1:
The detection device is divided into distinct functional modules: a detection chip with reaction chamber, a heating unit positioned beneath the chip, and a light path system. This segmentation allows independent optimization of each component, enabling precise temperature control without complicating the overall device architecture.
Solution Approach 2:
A transparent heating element or heating plate is introduced as an intermediary component between the light source and the detection chip. This intermediary enables thermal control while maintaining optical transparency, allowing excitation light to pass through without significant absorption or scattering.
2Measurement precision
If a heating section is added to maintain constant temperature, then the detection accuracy improves, but the light path may be interfered with causing temperature fluctuations or detection errors
Solution Approach 1:
The heating unit is positioned in a different spatial dimension (beneath the detection chip) rather than within the light path plane. This vertical arrangement allows thermal control from below while keeping the horizontal light path clear of heating components, eliminating optical interference.
Solution Approach 2:
A transparent heating element serves as an intermediary that transmits both heat and light simultaneously. This component enables thermal control while maintaining optical transparency, allowing excitation light to pass through without significant absorption or scattering.
3Productivity
If temperature is increased to 37 degrees for optimal reaction rates, then the primary and secondary reaction rates improve, but the fluorescence intensity may become unstable due to temperature sensitivity
Solution Approach 1:
A temperature sensor is integrated into the detection chip or heating unit to continuously monitor the reaction chamber temperature. This feedback signal is used by a control system to adjust the heating power, maintaining temperature within a narrow range around 37°C, thereby stabilizing both reaction rates and fluorescence intensity.
Solution Approach 2:
The system dynamically adjusts the heating parameter (power input) based on real-time temperature measurements. By changing the heating intensity in response to temperature fluctuations, the system maintains optimal reaction conditions while ensuring fluorescence stability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for highly sensitive and quantitative detection of target substances by maintaining a constant temperature, enhancing the reliability and accuracy of the detection process.
Implementation Method 1
a heating section that heats at least any one of the base body, the prism, and the metal film
Implementation Method 2
when the metal film is irradiated with excitation light through the prism at an angle at which surface plasmon resonance occurs, localized-field light can be generated on the surface of the metal film
Implementation Method 3
the fluorescent material used for labeling the captured detection target substance on the metal film is selectively excited, and the fluorescence emitted from the fluorescent material is observed
Data Source
AI summary
This detection device has a holder and a heating unit. The holder holds a detection chip that has the following: a prism that has an incidence surface and a film-formation surface; a metal film formed on said film-formation surface; trapping bodies laid out on the surface of said metal film; and a substrate that is laid out on the surface of the metal film, and together with the metal film, forms a liquid collection section in which a liquid is collected. The heating unit heats at least one of the substrate, the prism, and the metal film either while in contact therewith or without contacting same. Also, the heating unit is positioned so as to avoid the path that excitation light takes from an excitation-light emission unit to the abovementioned incidence surface.