SPR Detection Device Isolating Plasmon Scattered Light from Stray Light
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Solution Overview
Problem
In surface plasmon resonance fluorescence analysis, stray light generated by diffracted excitation light interferes with the detection of plasmon scattered light, making it difficult to accurately determine the incident angle of excitation light and resulting in noise in the measurement of the substance's presence or amount, which increases costs and reduces measurement performance.
Innovation Solution
A detection device and method that control the detection range of the detection unit to differentiate between plasmon scattered light and fluorescence emission, using a prism with a metal film and a capturing body to isolate the detection ranges for accurate plasmon scattered light detection without stray light interference.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If the excitation light is thinly beam-formed using a pinhole or slit to reduce cost and improve reaction efficiency, then the beam size is reduced and reaction efficiency is improved, but diffracted light is generated and stray light interferes with detection
Solution Approach 1:
The detection process is segmented into two distinct detection ranges: a first detection range for detecting plasmon scattered light at the irradiation spot, and a second detection range for detecting fluorescence from the reaction field. This segmentation allows the system to separately detect different types of light signals without mutual interference, resolving the contradiction between using beam-forming structures (which improve efficiency) and avoiding stray light interference.
Solution Approach 2:
The patent extracts and separates the detection of plasmon scattered light from the detection of fluorescence by assigning them to different detection ranges. The plasmon scattered light detection is performed in a first detection range centered on the irradiation spot, while fluorescence detection is performed in a second detection range corresponding to the reaction field. This extraction eliminates the interference between the two detection signals.
2Adaptability or versatility
If the detection range is expanded to detect both plasmon scattered light and fluorescence, then comprehensive detection is achieved, but stray light enters the detection range and creates noise
Solution Approach 1:
The detection range is segmented into two distinct regions: a first detection range for plasmon scattered light and a second detection range for fluorescence. This segmentation enables the system to maintain comprehensive detection capability while preventing stray light from contaminating either detection channel, thus preserving measurement accuracy.
Solution Approach 2:
Different detection ranges are assigned different qualities or functions: the first detection range is optimized for detecting plasmon scattered light with a specific angular distribution, while the second detection range is optimized for detecting fluorescence emission. This local differentiation of detection qualities allows comprehensive detection without cross-interference.
3Measurement precision
If the incident angle is adjusted to optimize plasmon resonance, then detection sensitivity is improved, but stray light detection makes it difficult to determine the optimum angle
Solution Approach 1:
The measurement process is segmented into two sequential phases: first, detecting plasmon scattered light in the first detection range to determine the optimum incident angle (enhancement angle); second, detecting fluorescence in the second detection range using the determined optimum angle. This segmentation allows accurate angle determination without stray light interference, thereby achieving both high sensitivity and accurate angle measurement.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate detection of plasmon scattered light without stray light interference, allowing for precise determination of the incident angle and improved measurement accuracy of the substance's presence or amount without increasing the detection chip's cost or deteriorating measurement performance.
Implementation Method 1
surface plasmon resonance (SPR)... when the metal film is irradiated with excitation light from the excitation light irradiation unit at an angle at which surface plasmon resonance occurs via the prism, localized field light (enhanced electric field) can be generated on the surface of the metal film
Implementation Method 2
the fluorescent substance that labels the substance to be detected captured on the metal film is excited by the localized field light, detecting the fluorescence emitted from the fluorescent substance allows the presence or amount of the substance to be detected to be detected
Implementation Method 3
a prism in which a metal film is disposed on a predetermined surface is used. Then, when the metal film is irradiated with excitation light from the excitation light irradiation unit at an angle at which surface plasmon resonance occurs via the prism
Data Source
AI summary
The present invention relates to providing a detection device that uses surface plasmon resonance to detect the presence or the amount of a substance to be detected, in which a detection unit can accurately detect scattered light without detecting stray light, and can accurately determine an irradiation angle of excitation light. This detection method uses the detection device that uses surface plasmon resonance to detect the presence or the amount of a substance to be detected. A detection range control unit controls a detection range of a detection unit so that a detection range utilized when the detection unit detects scattered light emitted from a metallic film and front a region on the metallic film differs from a detection range utilized when the detection unit detects fluorescence emitted front a fluorescent substance labeling the substance to be detected winch has been captured by a capturing body.