SPR Fluorescence Analysis Device Filter Segmentation
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Solution Overview
Problem
Existing surface plasmon resonance fluorescence analysis devices face challenges in maximizing detection sensitivity and accuracy due to differences between the incident angles for maximizing fluorescence and plasmon scattering light intensity, leading to increased device size and complexity when trying to optimize for enhanced angles.
Innovation Solution
Incorporating a scattering-light transmitting section at the excitation-light cut filter or filter holder to allow plasmon scattering light to pass through, enabling detection of the enhanced angle without significantly moving the excitation-light cut filter from the light path, and adjusting the filter's position to allow both fluorescence and scattering light to be detected efficiently.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the excitation-light cut filter is completely moved from the light path to determine the enhanced angle, then the detection sensitivity and accuracy are improved, but the device size increases
Solution Approach 1:
The excitation-light cut filter is segmented into two functional regions: a fluorescent transmitting region for fluorescence detection and a scattering-light transmitting section for plasmon scattering light detection. This segmentation allows both functions to coexist in the same filter structure, eliminating the need to completely move the filter and reducing device size while maintaining detection precision.
Solution Approach 2:
The excitation-light cut filter is designed with multi-functionality by incorporating both the fluorescent transmitting region and the scattering-light transmitting section. This universal design enables the single filter to serve dual purposes: blocking excitation light for fluorescence detection and transmitting plasmon scattering light for angle determination, thereby avoiding device enlargement.
2Measurement precision
If the incident angle is set to maximize fluorescence intensity, then detection sensitivity is improved, but the plasmon scattering light intensity is not maximized
Solution Approach 1:
The system dynamically switches between two detection modes by controlling the transmission characteristics of the excitation-light cut filter. In fluorescence detection mode, the filter transmits fluorescence while blocking excitation light. In plasmon scattering light detection mode, the scattering-light transmitting section allows plasmon scattering light to pass through. This dynamic control enables optimization of both fluorescence intensity and plasmon scattering light intensity at different incident angles.
Solution Approach 2:
The system changes the optical parameters of the excitation-light cut filter by adjusting the incident angle of excitation light. By varying the incident angle, the system can maximize either fluorescence intensity or plasmon scattering light intensity depending on the detection mode. The filter's transmission characteristics are modified through angle adjustment, enabling flexible optimization of detection parameters.
3Ease of operation
If a scattering-light transmitting section is added to the filter, then the enhanced angle can be determined without moving the filter, but the device complexity increases
Solution Approach 1:
The scattering-light transmitting section is merged with the excitation-light cut filter into a single integrated structure. This combining approach allows the filter to maintain a fixed position while providing both fluorescence transmission and plasmon scattering light transmission functions, avoiding the complexity of separate movable filter components and reducing overall device complexity.
Solution Approach 2:
Different regions of the excitation-light cut filter are assigned different local qualities: the fluorescent transmitting region has high transmission for fluorescence wavelengths, while the scattering-light transmitting section has high transmission for plasmon scattering light wavelengths. This local differentiation enables the single filter structure to handle multiple wavelengths and functions without increasing overall device complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for high-sensitivity, high-accuracy detection of target substances without significantly increasing the device's size, achieving downsizing and cost reduction while maintaining detection sensitivity and accuracy.
Implementation Method 1
Surface plasmon-field enhanced fluorescence spectroscopy (hereinafter abbreviated as 'SPFS') is known as a method which can detect a detection target substance with high sensitivity
Implementation Method 2
The captured detection target substance is then labeled by a fluorescent material (secondary reaction). In this state, when the metal film is irradiated with excitation light through the prism at an angle where SPR occurs, localized-field light can be generated on the surface of the metal film. With this localized-field light, the fluorescent material used for labeling the captured detection target substance on the metal film is selectively excited, and the fluorescence emitted from the fluorescent material is observed.
Implementation Method 3
an excitation-light cut filter that blocks excitation light but allows fluorescence to pass through the filter is provided before a light sensor that detects the fluorescence
Implementation Method 4
a scattering-light transmitting section disposed at the excitation-light cut filter or the filter holder and configured to allow plasmon scattering light emitted from the metal film to pass through the section
Data Source
AI summary
A surface plasmon fluorescence analysis device that has a chip holder, a light source, an angle adjustment unit, a light sensor, a filter holder, an excitation light cut filter, a scattered light transmission unit, a transmission adjustment unit, and a control unit. As seen in plan view, the area occupied by the scattered light transmission unit is arranged on the excitation light cut filter or on the filter holder and is smaller than the area of a fluorescence transmission region as seen in plan view.