SPR Hapten Detection via Reflected Light Mapping
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods for detecting haptens, such as ELISA, are not capable of providing real-time detection results on-site, which is a significant limitation for immediate contamination monitoring in water or food.
Innovation Solution
A high-sensitivity hapten detection method utilizing a SPR device to measure reflected light intensity from a sample solution containing a hapten, allowing for real-time concentration determination by mapping light intensity to concentration via a lookup table.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If ELISA method is used for hapten detection, then detection sensitivity is achieved, but real-time detection capability is lost
Solution Approach 1:
The patent replaces the mechanical/enzymatic reaction system of ELISA with an optical detection system based on surface plasmon resonance (SPR). The SPR device uses light reflection principles to detect hapten-antibody binding events in real-time without requiring enzymatic reactions or multiple washing steps, thereby achieving both high sensitivity and real-time detection capability.
Solution Approach 2:
The patent changes the detection parameter from enzymatic activity measurement (in ELISA) to optical reflection intensity measurement (in SPR). By monitoring the reflected light intensity changes caused by refractive index variations at the sensor surface during hapten-antibody binding, the system achieves real-time quantitative detection while maintaining high sensitivity.
2Measurement precision
If traditional detection methods are used, then detection accuracy is maintained, but on-site real-time monitoring capability is compromised
Solution Approach 1:
The patent replaces complex laboratory-based detection procedures with a simplified optical detection system that can be deployed on-site. The SPR device requires only sample addition and automatic optical measurement, eliminating the need for complex sample preparation, enzymatic reactions, and plate reading equipment, thereby enabling on-site real-time monitoring while maintaining detection accuracy.
Solution Approach 2:
The SPR-based detection system performs self-measurement by automatically detecting optical signal changes when sample is added to the sensor surface. The system requires minimal manual intervention beyond sample addition, as the optical detection and data processing are automated, making it suitable for on-site operation by non-specialist personnel.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables instantaneous and accurate detection of hapten concentrations, overcoming the limitations of traditional methods by providing real-time results and improving on-site monitoring capabilities.
Implementation Method 1
by using a SPR (surface plasmon resonance) device to detect a reflected light intensity caused by a sample solution containing a hapten
Implementation Method 2
detect a reflected light intensity caused by a sample solution containing a hapten
Data Source
AI summary
A high-sensitivity hapten detection method capable of deriving a real time hapten concentration by using a SPR device, in which different concentrations of a hapten in a sample solution result in different reflected light intensities on the SPR device, and by mapping a reflected light intensity, via a lookup table, to a corresponding concentration, a detection result of the concentration of the hapten in the sample solution can be instantly provided.