Spray-Dried Beta-Galactosidase with Maltodextrin
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Solution Overview
Problem
Current commercial lactase products formulated in polyols like glycerol are physically stable but perturb the transgalactosylation activity of β-galactosidases, leading to unwanted galactosyl-glycerol production instead of desired galacto-oligosaccharides, and lack stability and efficiency in milk-based products.
Innovation Solution
A spray-dried composition of β-galactosidase with maltodextrin or sodium chloride as carriers, which maintains transgalactosylation activity and physical stability, allowing for efficient production of galacto-oligosaccharides even at low lactose concentrations in milk-based products.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If lactase products are formulated in polyols like glycerol, then physical stability is improved, but transgalactosylation activity is perturbed and unwanted galactosyl-glycerol is produced
Solution Approach 1:
The patent uses spray-dried carriers (maltodextrin, sodium chloride, or starch) as intermediary substances to formulate the enzyme. These carriers provide physical stability without interfering with the enzyme's transgalactosylation activity, unlike polyols which act as harmful intermediaries that divert the enzyme's activity to unwanted products.
Solution Approach 2:
The patent changes the formulation parameters by eliminating polyols and using alternative carriers. The enzyme is formulated in a dry spray-dried state rather than in polyol solutions, fundamentally changing the physical and chemical environment to preserve transgalactosylation activity while maintaining stability.
2Stability of the object's composition
If current commercial formulations are used, then physical stability is achieved, but efficiency in milk-based products is insufficient
Solution Approach 1:
The spray-dried carriers serve as inert intermediaries that support the enzyme without interfering with its function in milk-based products. This allows the enzyme to efficiently perform transgalactosylation on lactose in milk without the inhibitory effects of polyol formulations.
3Duration of action of stationary object
If polyol formulations are used, then shelf life is extended, but transgalactosylation activity is reduced
Solution Approach 1:
The patent changes the formulation from liquid polyol-based to dry spray-dried powder form. This parameter change extends shelf life through reduced moisture content while simultaneously preserving transgalactosylation activity by eliminating polyol interference with the enzyme's catalytic function.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The spray-dried composition retains the transgalactosylation activity of β-galactosidase, ensuring the production of desired galacto-oligosaccharides while providing physical stability, addressing the limitations of existing formulations.
Implementation Method 1
spray-dried composition comprising a polypeptide which is a β-galactosidase having transgalactosylating activity
Data Source
AI summary
A spray-dried composition comprising an enzyme which is a β-galactosidase having transgalactosylating activity and a maltodextrin and/or sodium chloride.