Spray-Dried Human Milk Oligosaccharide Powder Production

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Solution Overview

Problem

Current methods for producing human milk oligosaccharides (HMOs) face challenges such as the use of organic solvents, which are costly, environmentally harmful, and not suitable for human consumption, especially during the final stages of recovery processes.

Innovation Solution

A process for manufacturing a spray-dried powder containing a mixture of structurally distinct HMOs, along with at least one monosaccharide like L-fucose or N-acetylneuraminic acid, is developed. This process avoids the use of organic solvents and results in a solid, low-water product that is less susceptible to microbial contamination.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Manufacturing precision

If organic solvents are used in the final stages of HMO recovery processes, then HMOs can be obtained in solid form, but the process becomes costly, environmentally harmful, and unsuitable for human consumption

Engineering Contradiction:
Improvesolid form recoveryVSAvoidorganic solvent contamination
Core Design Contradiction:
Manufacturing precisionVSObject-affected harmful factors

Solution Approach 1:

The patent extracts and removes organic solvents from the HMO recovery process entirely. Instead of using organic solvents for precipitation and crystallization, the process employs aqueous-based methods including ultrafiltration, diafiltration, and spray-drying to obtain HMOs in solid form, thereby eliminating solvent contamination while maintaining product quality

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent changes the physical and chemical parameters of the recovery process by operating at controlled temperatures (e.g., 20-80°C for ultrafiltration, 120-150°C for spray-drying) and using aqueous buffers instead of organic solvents. This parameter transformation allows solid HMO recovery without introducing harmful organic contaminants

Inventive Principle:
Principle #35Parameter changes

2Ease of manufacture

If HMOs are produced as aqueous solutions, then the process is environmentally friendly and cost-effective, but the product is susceptible to microbial contamination and has poor storage characteristics

Engineering Contradiction:
Improvecost-effectivenessVSAvoidmicrobial contamination risk
Core Design Contradiction:
Ease of manufactureVSReliability

Solution Approach 1:

The patent employs phase transition from liquid to solid state through spray-drying. The aqueous HMO solution is atomized into droplets and rapidly dried, transforming the product into a stable powder form that is resistant to microbial contamination while maintaining the cost-effectiveness of aqueous-based processing

Inventive Principle:
Principle #36Phase transitions

Solution Approach 2:

The patent replaces chemical preservation methods with physical drying (spray-drying) to prevent microbial contamination. The mechanical/thermal energy input during spray-drying removes water activity, creating an environment where microbes cannot grow, thus ensuring product reliability without adding chemical preservatives

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Stability of the object's composition

If HMOs are spray-dried to produce solid powder, then the product is less hygroscopic and remains flowable for longer, but the process requires careful control of drying parameters

Engineering Contradiction:
ImproveflowabilityVSAvoiddrying process control
Core Design Contradiction:
Stability of the object's compositionVSDevice complexity

Solution Approach 1:

The patent optimizes spray-drying parameters including inlet temperature (120-150°C), outlet temperature (40-80°C), and feed concentration (20-50% w/v) to achieve the desired balance between flowability and stability. These controlled parameter changes produce a powder with appropriate moisture content and particle characteristics without excessive process complexity

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The process effectively produces a stable, solid HMO product that is less hygroscopic and remains flowable for longer, reducing the risk of contamination and improving handling and storage characteristics, while also being environmentally friendly and cost-effective.

Implementation Method 1

spray-dried powder containing a mixture of structurally distinct HMOs

Methodology Applied
Scientific EffectEvaporation: Evaporation

Implementation Method 2

spray-dried powder containing a mixture of structurally distinct HMOs

Methodology Applied
Scientific EffectPhase change: Phase Change

Data Source

PatentEP3524067B1Spray-dried mixture of human milk oligosaccharides
Publication Date: 2025.04.02 CHR HANSEN HMO GMBH
  • EP3524067B1 patent drawingFigure 1~2
  • EP3524067B1 patent drawingFigure 3~4
  • EP3524067B1 patent drawingFigure 5~6

AI summary

Disclosed are spray-dried powders containing a mixture of structurally distinct human milk oligosaccharides, methods for the production of said spray-dried powder, its use for the manufacture of nutritional compositions, and nutritional compositions containing said spray-dried powder.