Squid Allergy Diagnostic Kit Using MLC1 and MLC2 Antibody Capture
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Solution Overview
Problem
Current diagnostic methods for seafood allergies, particularly squid allergy, are limited in specificity and sensitivity, making it difficult to distinguish between squid and other seafood allergies, and there is a need for more effective reagents and tests to diagnose and treat seafood allergies effectively.
Innovation Solution
A diagnostically useful carrier is developed to specifically capture antibodies to squid myosin light chain 1 (MLC1) or squid MLC2, or their variants, along with squid tropomyosin (TM), using a kit that includes a microtiter plate or line blot, and a method to detect IgE antibodies, allowing for the differential diagnosis of squid allergy from other seafood allergies.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional IgE detection tests are used for seafood allergy diagnosis, then the test can detect IgE antibodies to common seafood allergens, but the test lacks specificity to distinguish squid allergy from other seafood allergies
Solution Approach 1:
The patent segments the seafood allergy diagnosis by introducing specific squid allergen components (MLC1, MLC2, and TM proteins) as separate detection targets. This segmentation allows the test to specifically identify squid allergy versus other seafood allergies by detecting IgE antibodies against these particular squid proteins, thereby improving diagnostic precision without requiring entirely new test systems.
Solution Approach 2:
The diagnostic kit is designed with multi-functionality to detect multiple squid allergen components (MLC1, MLC2, and TM) simultaneously within a single test platform. This universal approach allows the same kit to differentiate various types of seafood allergies while maintaining a unified diagnostic system, avoiding the need for multiple separate tests.
2Reliability
If comprehensive seafood allergen testing is performed to identify specific allergens, then the diagnostic accuracy improves, but the time and resources required for testing increase
Solution Approach 1:
The patent merges the detection of multiple squid allergen components (MLC1, MLC2, and TM proteins) into a single integrated diagnostic kit. By combining these detection capabilities in one test, the system achieves comprehensive allergy diagnosis accuracy while reducing the time and resources that would be required if separate tests were conducted for each allergen component.
Solution Approach 2:
The diagnostic approach uses preliminary identification of squid-specific allergen components (MLC1, MLC2, TM) to guide the testing process. By pre-selecting and preparing these specific squid allergen targets, the test can quickly and accurately determine whether a patient's allergy is specifically to squid, avoiding the need for broader, more time-consuming seafood allergen screening.
3Measurement precision
If specific squid allergen components are targeted for detection, then the diagnostic specificity for squid allergy improves, but the complexity of the diagnostic kit increases
Solution Approach 1:
The diagnostic kit applies local quality by focusing detection capabilities on specific squid allergen components (MLC1, MLC2, and TM proteins) rather than attempting to detect all seafood allergens. This localized approach to quality enhances squid allergy detection specificity while keeping the kit design manageable by concentrating on a defined set of target proteins rather than a broad spectrum of allergens.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enables accurate detection of antibodies to squid MLC1 and MLC2, enhancing the diagnostic capability for squid allergy and differentiating it from other seafood allergies, thereby improving the diagnosis and treatment of seafood allergies.
Implementation Method 1
a means for specifically capturing an antibody to a polypeptide from the group comprising squid MLC1 or squid MLC2 or a variant
Data Source
AI summary
A diagnostically useful carrier includes a means for specifically capturing an antibody to a polypeptide from the group including squid MLC1 or squid MLC2 or a variant thereof in a sample from a subject. A method includes the step detecting in a sample from a subject the presence or absence of an antibody to squid MLC1 or squid MLC2. The polypeptide or the carrier or a polypeptide binding specifically to an IgE antibody from the sample of a patient to squid MLC1 or squid MLC2 are useful for the manufacture of a diagnostic kit, preferably for the diagnosis of allergy.