Single-Stranded Binding Moieties Prevent Particle Aggregation
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Solution Overview
Problem
Nucleic acid sequencing technologies face challenges due to particle aggregation, which reduces sequencing quality and stability of nucleic acid molecules coupled to particles, affecting diagnostic and research applications.
Innovation Solution
The method involves processing particles by immobilizing nucleic acid molecules with single-stranded binding moieties, such as SSB proteins, to prevent aggregation, ensuring that nucleic acid molecules remain stable and non-aggregated, even during storage and sequencing processes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If nucleic acid molecules are coupled to particles for sequencing, then sequencing capability is enabled, but particle aggregation occurs reducing sequencing quality
Solution Approach 1:
A blocking nucleic acid molecule is introduced as an intermediary between the nucleic acid molecules coupled to particles. This blocking molecule prevents direct association between nucleic acid molecules on different particles, thereby eliminating particle aggregation while maintaining sequencing capability. The blocking molecule acts as a physical barrier that stops harmful interactions without interfering with the intended sequencing process.
2Duration of action of stationary object
If particles are stored for long-term use, then storage duration is extended, but nucleic acid molecules become unstable and aggregate
Solution Approach 1:
The blocking nucleic acid molecule is applied in advance during particle preparation, before storage begins. This preliminary protective measure prevents nucleic acid association and aggregation from occurring during long-term storage. By establishing the blocking mechanism beforehand, the system maintains nucleic acid stability throughout the storage period without requiring continuous intervention.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly reduces particle aggregation, improving sequencing quality and enabling long-term storage and analysis of nucleic acid samples by maintaining the integrity of nucleic acid molecules, thereby enhancing diagnostic and research capabilities.
Implementation Method 1
contacting the first nucleic acid molecule with a first single-stranded binding moiety under conditions sufficient to couple the first single-stranded binding moiety to the first single-stranded portion of the first nucleic acid molecule
Data Source
AI summary
The present disclosure provides methods, systems, compositions, and kits for reducing particle aggregation and improving sequencing quality. A method for processing particles may comprise the use of a first buffer solution or a second buffer solution. A method for processing particles may comprise the use of a single-strand binding moiety such as a single-strand binding protein.