SSEA-3+ Pluripotent Stem Cell Isolation Without Tumorigenicity

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Solution Overview

Problem

Current methods for obtaining pluripotent stem cells from human tissues require artificial manipulation, such as gene introduction, and are limited by tumorigenicity and inefficient differentiation capabilities.

Innovation Solution

Isolation of SSEA-3-positive pluripotent stem cells from body tissues using SSEA-3 and CD105 as markers, which can differentiate into all three germ layers without tumorigenicity and are resistant to stress, allowing for direct isolation and use in tissue repair.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If artificial manipulation methods (gene introduction) are used to obtain pluripotent stem cells, then pluripotent stem cells can be generated, but tumorigenicity occurs and limits clinical application

Engineering Contradiction:

Improvesafety of pluripotent stem cellsVSAvoidtumorigenicity

Core Design Contradiction:

ReliabilityVSObject-generated harmful factors

Solution Approach 1:

The invention extracts and isolates pluripotent stem cells directly from adult body tissues (such as bone marrow, adipose tissue, or umbilical cord) without introducing exogenous genes. This extraction approach obtains pluripotent cells through natural isolation rather than artificial reprogramming, thereby eliminating tumorigenicity while maintaining pluripotency capabilities

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

Instead of following the conventional approach of converting somatic cells into pluripotent cells through gene introduction (iPS method), the invention inverts the approach by directly isolating pluripotent stem cells that naturally exist in adult tissues. This reversal eliminates the need for artificial manipulation and associated tumorigenic risks

Inventive Principle:

Principle #13The other way round (Inversion)

2Adaptability or versatility

If bone marrow stromal cells are used for tissue repair, then differentiation into various cell types is possible, but the cells responsible for repair remain unclear and require complex induction systems

Engineering Contradiction:

Improvedifferentiation capabilityVSAvoidcomplexity of induction system

Core Design Contradiction:

Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The isolated pluripotent stem cells possess inherent self-differentiation capability without requiring external induction systems. When transplanted into damaged tissues, these cells automatically differentiate into the appropriate cell types needed for repair, eliminating the need for complex gene transfer or compound stimulation protocols

Inventive Principle:

Principle #25Self-service

Solution Approach 2:

The pluripotent stem cells exhibit universal differentiation potential across multiple tissue types (ectodermal, mesodermal, and endodermal lineages) while maintaining a single cell population. This multi-functionality allows one cell type to serve multiple repair purposes without requiring separate induction systems for different tissue types

Inventive Principle:

Principle #6Universality (Multi-functionality)

Data Source

PatentUS11261426B2Pluripotent stem cell that can be isolated from body tissue

Publication Date: 2022.03.01 MUSECELL INNOVATIONS PTE LTD

AI summary

Objects of the present invention are to provide a method for directly obtaining pluripotent stem cells which do not have tumorigenic property from body tissue and the thus obtained pluripotent stem cells. The present invention relates to SSEA-3 (+) pluripotent stem cells that can be isolated from body tissue.