SSR Marker Panel and Primer Kit for Procapra Individual Identification

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Solution Overview

Problem

There is a lack of effective methods for individual identification of Procapra przewalskii using simple sequence repeats (SSR) molecular markers, despite the importance of this species for conservation efforts.

Innovation Solution

Development of a combination of 4-base SSR molecular markers and primer pairs, including PR-6, PR-8, PR-12, PR-22, PR-25, PR-30, PR-40, PR-58, PR-63, PR-69, PR-71, PR-72, PR-85, PR-86, and PR-97, for individual identification, utilizing whole-genome sequencing data and capillary electrophoresis for analysis.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If mitochondrial molecular markers are used for population genetic diversity research, then population-level analysis can be conducted, but individual identification capability is insufficient

Engineering Contradiction:

Improveindividual identification accuracyVSAvoidmarker system complexity

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent segments the genetic analysis into two distinct components: mitochondrial molecular markers for population-level genetic diversity research and nuclear SSR markers for individual identification. This segmentation allows each marker type to be optimized for its specific function, with SSR markers providing the necessary polymorphism for individual differentiation while mitochondrial markers maintain their utility for population studies.

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The patent creates a multi-functional marker system where SSR markers serve dual purposes: they enable individual identification through their high polymorphism and can also contribute to population genetic diversity analysis. This universal approach allows a single marker set to address multiple research needs, reducing the overall complexity of the analytical framework.

Inventive Principle:

Principle #6Universality (Multi-functionality)

2Measurement precision

If SSR markers are developed based on whole-genome sequencing data, then individual identification accuracy is improved, but the development process and initial investment increase

Engineering Contradiction:

Improveindividual identification precisionVSAvoidmarker development ease

Core Design Contradiction:

Measurement precisionVSEase of manufacture

Solution Approach 1:

The patent performs preliminary whole-genome sequencing and SSR marker development during the research preparation phase, creating a ready-to-use marker system before field studies begin. This preliminary action includes identifying SSR loci, designing primers, and validating markers for individual identification, thereby eliminating these complex steps from future routine studies and improving ease of manufacture for subsequent applications.

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

Once SSR markers are developed and validated using whole-genome sequencing data, the patent creates standardized primer sets and protocols that can be copied and applied to multiple Procapra przewalskii studies. This copying approach allows other researchers to use the same marker system without repeating the expensive and time-consuming whole-genome sequencing process, thereby improving ease of manufacture while maintaining high measurement precision.

Inventive Principle:

Principle #26Copying

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The developed markers provide high accuracy and polymorphism for individual identification, population genetic diversity detection, and kinship research, with a success rate of 1/10,000 for individual identification, consistent with the actual population count.

Implementation Method 1

the SSR molecular markers are sequentially amplified by following primer pairs

Methodology Applied

Scientific EffectPolymerase chain reaction:

Implementation Method 2

utilizing whole-genome sequencing data and capillary electrophoresis for analysis

Methodology Applied

Scientific EffectCapillary electrophoresis: Capillary Electrophoresis

Data Source

PatentUS20260071274A1Combination of simple sequence repeats (SSR) molecular marker, primer combination, kit for procapra przewalskii, and use

Publication Date: 2026.03.12 NORTHWEST INST OF PLATEAU BIOLOGY CHINESE ACAD OF SCI

AI summary

The present disclosure provides a combination of single sequence repeat (SSR) molecular marker for Procapra przewalskii, a primer combination, a kit, and use, and relates to the technical field of molecular biology. In the present disclosure, design of SSR primers and screening of SSR loci are conducted based on a whole-genome sequencing data of Procapra przewalskii. 26 pairs of obtained SSR primers can stably amplify a target product, and are highly polymorphic, and can be used for population genetic diversity detection, population genetic structure analysis, evolution, and kinship research of the Procapra przewalskii. A combination of polymorphic SSR molecular marker of Procapra przewalskii selected can be used for individual identification of the Procapra przewalskii with a high accuracy. The experimental results show that the combination of SSR molecular marker of Procapra przewalskii can meet the needs of individual identification.