Stabilizer for Color Developing Agent Suppressing Spontaneous Oxidation
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Solution Overview
Problem
Existing hydrogen peroxide color developing agents for chemical detection have low stability and are prone to spontaneous color development, limiting their application due to interference from components like hemoglobin and bilirubin, and previous stabilizers increase reagent blank, hindering practical use.
Innovation Solution
A stabilizer composition comprising a reducing substance such as sodium sulfite or sodium thiosulfate and a weakly acidic buffer with a pH of 3.8-6.2 is used to stabilize phenothiazine or triphenylmethane color developing agents, suppressing spontaneous color development and maintaining their reactivity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If a stabilizer containing azo dye is used to stabilize a color developing agent, then the stability of the color developing agent is improved, but the overall blank of the reagent increases, hindering practical application
Solution Approach 1:
The patent extracts and removes the azo dye component from the stabilizer system. Instead of using a stabilizer containing azo dye (which causes high blank), the invention uses a stabilizer free of azo dyes (such as sodium sulfite, sodium thiosulfate, or 1-mercaptoglycerol in weakly acidic buffer), thereby eliminating the harmful blank effect while maintaining the stabilizing function.
Solution Approach 2:
The patent introduces alternative stabilizing substances (sodium sulfite, sodium thiosulfate, 1-mercaptoglycerol) as intermediary compounds that can stabilize the color developing agent without the harmful side effects of azo dyes. These intermediaries perform the stabilizing function through different chemical mechanisms (reducing properties) that do not interfere with the detection system.
2Measurement precision
If a novel hydrogen peroxide color developing agent is used to improve sensitivity and reduce interference, then the detection performance is improved, but the stability of the agent decreases, causing easy spontaneous color development
Solution Approach 1:
The patent applies preliminary anti-action by adding a stabilizer (sodium sulfite, sodium thiosulfate, or 1-mercaptoglycerol in weakly acidic buffer) to the color developing agent formulation before use. This stabilizer preemptively counteracts the spontaneous oxidation and color development of the novel hydrogen peroxide color developing agent, allowing it to remain stable during storage and handling while maintaining its high sensitivity and anti-interference properties when needed for detection.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The stabilizer effectively prolongs the preservation time of color developing agents while maintaining their color developing ability, preventing redox interference and ensuring stable preservation without affecting the agent's properties, thus overcoming the limitations of previous stabilizing methods.
Implementation Method 1
The stabilizer includes a reducing substance and a weakly acidic buffer... suppressing spontaneous color development and maintaining their reactivity
Data Source
AI summary
Disclosed in the present invention are a stabilizer for a color developing agent and application thereof, an application of a composition in preparation of the stabilizer, and a kit. A stabilizer for a color developing agent is provided in the present invention. The stabilizer includes a reducing substance and a weakly acidic buffer, and the weakly acidic buffer has a pH of 3.8-6.2. The reducing substance includes one or more of sodium sulfite, sodium bisulfite, sodium thiosulfate, or 1-mercaptoglycerol. The color developing agent includes one or two of a phenothiazine color developing agent or a triphenylmethane color developing agent. The color developing agent can be stably preserved by using the stabilizer. A method for stably preserving a color developing agent includes dissolving the color developing agent in the stabilizer.