Stable Aqueous Lipase Enzyme Compositions
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Solution Overview
Problem
Enzymes used in cleaning applications are unstable in aqueous solutions due to incompatible chemistries and autolysis, leading to reduced effectiveness and limitations in their use, particularly in the foodservice industry where stubborn soils require effective cleaning products without aggressive chemicals.
Innovation Solution
A stable enzyme stabilization system is achieved by using specific ratios of acid to amine, combined with nonionic surfactants and solvents, which maintains enzyme activity even in the presence of other ingredients, forming a stable enzyme system suitable for cleaning applications.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If enzymes are used in aqueous cleaning solutions, then cleaning effectiveness is improved, but enzyme stability deteriorates due to incompatible chemistries and autolysis
Solution Approach 1:
The patent introduces a stabilizing system comprising specific ingredients (surfactants, solvents, and other compatible chemicals) that act as intermediaries between the enzyme and the aqueous cleaning solution. These stabilizers create a protective environment that allows the enzyme to function effectively while preventing denaturation and autolysis, thus resolving the contradiction between cleaning effectiveness and enzyme stability
Solution Approach 2:
The patent optimizes multiple parameters including pH range (5.5-7.5), temperature conditions, and concentrations of stabilizing ingredients to create optimal conditions for enzyme stability. By carefully controlling these parameters, the enzyme maintains its activity and stability in the aqueous cleaning solution, resolving the contradiction between effectiveness and stability
2Reliability
If boric acid or borate stabilization systems are used, then enzyme stability is improved, but regulatory restrictions worsen in certain countries
Solution Approach 1:
The patent replaces boric acid/borate systems with alternative stabilizing ingredients that achieve the same enzyme stability function without regulatory restrictions. This substitution maintains enzyme stability while improving regulatory compatibility and adaptability across different countries and markets
Solution Approach 2:
The patent uses alternative stabilizing ingredients that are safer and more universally acceptable than boron-containing compounds. These alternatives may have different stability profiles but provide sufficient enzyme protection without the regulatory burdens, allowing broader application and market access
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system retains at least 15% to 45% of initial enzyme activity after 21 days at 40°C, ensuring effective cleaning performance and antimicrobial properties, particularly in removing food soils and preventing bacterial growth in the foodservice industry.
Implementation Method 1
preferred ratios of acid to amine are effective at stabilizing enzymes
Implementation Method 2
preferred ratios of acid to amine are effective at stabilizing enzymes
Implementation Method 3
Nonionic surfactants and solvent also positively contribute to enzyme stability
Implementation Method 4
Nonionic surfactants and solvent also positively contribute to enzyme stability
Implementation Method 5
Nonionic surfactants and solvent also positively contribute to enzyme stability
Data Source
AI summary
The disclosure relates to an enzyme stabilization system, compositions with the enzyme stabilization system, and methods of using the enzyme composition. Preferred ratios of acid to amine are effective at stabilizing enzyme. Optional nonionic surfactants and solvents also positively contribute to enzyme stability. The compositions are useful in cleaning applications.