Stable I2SCN- and I(SCN)2- Ion Composition via Enzymatic Oxidation
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Solution Overview
Problem
Existing antimicrobial compositions using lactoperoxidase (LP) and thiocyanate ions suffer from instability and reduced antimicrobial activity over time, particularly when stored with oxygen, and often form hypothiocyanite ions (OSCN-) that are not as effective as other species like I2SCN- and I(SCN)2-, which are difficult to stabilize and maintain in traditional oxidation reactions.
Innovation Solution
A stable composition is achieved by enzymatically oxidizing a halide thiocyanate mixture in the presence of lactoperoxidase, specifically controlling the reaction conditions to produce predominantly I2SCN- and I(SCN)2- ions without forming OSCN-, and incorporating additional compounds like lactoferrin, lysozyme, or growth factors for enhanced stability and activity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If traditional chemical oxidation methods are used to produce antimicrobial compounds from thiocyanate ions and halogens, then the production process is simple, but the stability of the resulting composition is poor and antimicrobial activity decreases over time
Solution Approach 1:
The patent introduces lactoperoxidase enzyme as an intermediary catalyst to mediate the oxidation reaction between thiocyanate ions and halogens. This enzymatic mediator enables the formation of stable I2SCN- and I(SCN)2- ions while maintaining simple reaction conditions, resolving the contradiction between composition stability and process complexity
Solution Approach 2:
The patent changes the reaction parameters by using enzymatic oxidation instead of traditional chemical oxidation, controlling the pH and temperature conditions to optimize the formation of stable iodine-thiocyanate complex ions. This parameter change transforms the oxidation process to produce highly stable antimicrobial compositions
2Reliability
If the composition is stored in the presence of oxygen, then the composition remains accessible, but the bactericidal activity decreases significantly over time
Solution Approach 1:
The patent converts the harmful effect of oxygen, which normally causes degradation, into a beneficial feature by designing a system where oxygen can be present during storage without compromising activity. The enzymatic system and stable I2SCN- / I(SCN)2- ion complexes are specifically engineered to maintain bactericidal activity even in the presence of oxygen over extended storage periods
3Reliability
If hypothiocyanite ions (OSCN-) are formed as the main oxidation product, then the reaction follows traditional pathways, but the antimicrobial efficacy is limited compared to I2SCN- and I(SCN)2- ions
Solution Approach 1:
The patent changes the reaction parameters by using lactoperoxidase-catalyzed oxidation under controlled conditions to selectively produce I2SCN- and I(SCN)2- ions instead of the traditional OSCN- ions. This parameter change in the oxidation pathway dramatically improves antimicrobial efficacy while maintaining ease of manufacture through the enzymatic process
Solution Approach 2:
The patent replaces traditional chemical oxidation mechanisms with an enzymatic oxidation system using lactoperoxidase. This substitution of the oxidation mechanism enables selective formation of highly efficacious I2SCN- and I(SCN)2- ions while keeping the manufacturing process simple and controllable
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The resulting composition is highly stable, maintaining over 80% antimicrobial activity for months without the formation of OSCN-, and exhibits superior antimicrobial efficacy against a broad spectrum of pathogens, including bacteria, viruses, and fungi, with enhanced stability and prolonged effectiveness.
Implementation Method 1
oxidation of thiocyanate ion in the presence of a halogen, said oxidation being catalyzed using particular enzymes called oxidoreductases, and more precisely peroxidases, the preferred being lactoperoxidase
Implementation Method 2
said oxidation being catalyzed using particular enzymes called oxidoreductases
Implementation Method 3
H2O2 + SCN- → OSCN- + H2O
Data Source
Figure 1
Figure 2
Figure 3a
AI summary
The invention relates to a stable composition obtained by enzymatic oxidation of a halide-thiocyanate mixture including at least one ion selected from the group consisting of I2SCN" ions and I(SCN)2 ions, said composition being free of hypothiocyanite ions. [0002] In one embodiment, the composition according to the invention further comprises iodine thiocyanate (ISCN). [0003] The invention also relates to the method for preparing said stable composition and the uses thereof.