Stackable Pupal Containers for Automated Inoculation Handling

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Solution Overview

Problem

Existing methods for transporting and storing insect pupae for recombinant protein production are inefficient, time-consuming, and prone to cross-contamination, especially in automated processes, due to the use of reusable plastic matrices and manual handling.

Innovation Solution

A stackable container with a tray and lid design, featuring wells and openings for pupae, interlocking mechanisms, and ventilation passageways, along with an RFID tag for automated tracking and inoculation, ensuring secure, efficient, and controlled transportation and storage.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Ease of manufacture

If reusable plastic matrices are used for storing and transporting pupae, then the container can be reused, but manual handling is required and cross-contamination occurs between different vectors

Engineering Contradiction:
ImprovereusabilityVSAvoidmanual handling
Core Design Contradiction:
Ease of manufactureVSExtent of automation

Solution Approach 1:

The patent employs disposable plastic matrices with single-use lids instead of reusable containers. Each matrix is designed to be used once and then discarded, eliminating the need for cleaning and sterilization between different vector infections. This disposable approach enables fully automated handling since each single-use container is sealed and pre-prepared, removing manual intervention requirements while preventing cross-contamination between different recombinant protein productions.

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

2Ease of operation

If manual allocation of pupae is used, then flexibility in handling is maintained, but the process is time-consuming and cannot be automated

Engineering Contradiction:
ImproveflexibilityVSAvoidprocessing speed
Core Design Contradiction:
Ease of operationVSProductivity

Solution Approach 1:

The patent segments the pupae storage system into individual compartments within the plastic matrix, with each compartment holding a single pupa. This segmentation allows for automated robotic insertion and removal of entire matrices without manual handling of individual pupae. The standardized segmented structure enables programming of automated robots to process multiple containers simultaneously, dramatically increasing productivity while maintaining operational simplicity through the modular design.

Inventive Principle:
Principle #1Segmentation

3Quantity of substance

If stackable containers are used for transportation, then space efficiency and security are improved, but the container structure becomes more complex

Engineering Contradiction:
Improvespace utilizationVSAvoidcontainer structure
Core Design Contradiction:
Quantity of substanceVSDevice complexity

Solution Approach 1:

The patent implements a nested container system where smaller individual pupa matrices are placed inside a larger outer container. The matrices are designed to nest within each other when empty, and the lid assembly nests within the matrix structure. This nesting capability maximizes space utilization during transportation and storage, allowing multiple containers to be compacted into a single shipping unit. The nested design achieves high space efficiency without requiring complex mechanical structures, as the nesting is achieved through simple geometric interlocking of the plastic components.

Inventive Principle:
Principle #7Nested doll (Nesting)

Data Source

PatentUS12402612B2Container for transporting and inoculating pupae
Publication Date: 2025.09.02 ALTERNATIVE GENE EXPRESSION
  • US12402612B2 patent drawing
  • US12402612B2 patent drawing
  • US12402612B2 patent drawing

AI summary

The invention refers to a container (1) that can be used for storing, transporting and for inoculating silk-free pupae. The container (1) includes a tray (2) having a flat surface (5) and a plurality of wells (4) formed on the surface (5), wherein each well (4) is configured for accommodating a pupa (8). The container (1) also includes a lid (3) having a plurality of openings (6), wherein the tray (2) and the lid (3) are configured to be coupled to each other, such that the lid (3) is placed on the flat surface (5), at least partially, closing the wells (4). The wells (4) and the openings (6) are arranged, such that when the tray (2) and the lid (3) are coupled together, each well (4) is accessible through one of the plurality of openings (6). The container is stackable for an optimum and cost-efficient secure transportation.