Mesenchymal Stem Cell Preparation from Adipose Tissue
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for cosmetic procedures using autologous fat injections require multiple interventions to achieve long-term results due to low retention rates of injected fat, typically only 10-20% remaining in the body after a single dose, necessitating repeated procedures.
Innovation Solution
A method for purifying and obtaining a mesenchymal stem cell preparation from fatty tissue involves centrifugation, collagenase digestion of 40% of the fat, and mixing with 60% undigested fat to create a stem cell-enriched preparation for injection, optimizing growth and retention at the application site.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional autologous fat injection is performed, then the procedure is simple and uses the patient's own fat, but only 10-20% of the injected fat remains permanently requiring multiple procedures
Solution Approach 1:
The patent extracts mesenchymal stem cells from a portion of the harvested fat through enzymatic digestion with collagenase. This extracted stem cell fraction is then combined with undigested fat, creating a preparation with enhanced regenerative capacity that significantly improves fat retention rates compared to conventional direct injection methods.
Solution Approach 2:
The patent performs preliminary processing of the harvested fat by separating it into digested and undigested portions before injection. The digested portion undergoes enzymatic treatment to release stem cells, which are then mixed with the undigested fat. This preliminary preparation enhances the survival and integration of injected fat cells, reducing the need for repeat procedures.
2Reliability
If 100% of harvested fat is used for injection without processing, then the procedure is quick and simple, but fat retention is poor and requires repeated interventions
Solution Approach 1:
The patent divides the harvested fat into two segments: a digested portion (treated with collagenase to release stem cells) and an undigested portion (maintained in its native state). This segmentation allows the preparation to combine the regenerative benefits of stem cells with the structural integrity of intact fat cells, achieving both improved retention and controlled complexity.
Solution Approach 2:
The patent applies different treatments to different portions of the fat: one portion undergoes enzymatic digestion to extract stem cells, while another portion remains undigested to provide structural support. This local differentiation of treatment quality optimizes both the regenerative capacity and the physical properties of the injection material.
3Quantity of substance
If collagenase digestion is performed on all fat, then maximum stem cells are extracted, but the structural integrity and survival of fat cells is compromised
Solution Approach 1:
The patent applies collagenase digestion to only a portion (e.g., 50%) of the harvested fat rather than treating all of it. This partial action extracts sufficient stem cells to enhance the preparation's regenerative capacity while leaving the remaining fat intact to maintain structural integrity and cell survival. The optimal ratio balances stem cell quantity with tissue strength.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly increases the retention of fatty tissue at the injection site, with 30-80% remaining permanently, often achieving the desired effect in a single intervention, unlike conventional methods which require multiple procedures.
Implementation Method 1
incubating 40% of the centrifuged fat with collagenase at 30 ° to 40 ° C, preferably at 37 ° C, for 10 - 45 min
Implementation Method 2
separating the fat of a provided fatty tissue from excess water, oil and / or blood by centrifugation
Data Source
AI summary
The present invention relates to a method for purifying and obtaining a mesenchymal stem cell preparation from adipose tissue for tissue injection, comprising the steps of: a) providing adipose tissue, b) separating the fat from excess water, oil, and/or blood by centrifugation, c) dividing the centrifuged fat from step b) and incubating 40% of the centrifuged fat with collagenase and other connective tissue-digesting enzymes at 30° to 45°C, preferably 30° to 37°C, for 10 to 45 min, preferably for 20 to 40 min, d) isolating mesenchymal stem cells from the digested fat by centrifugation, e) mixing the isolated stem cells from step d) with the undigested fat from step c), wherein the proportion of undigested fat is 60%.